RASSF1 ELISA Kit

Catalog No. ABIN5658354

Product Details RASSF1 ELISA Kit

Target: RASSF1 (Ras Association (RalGDS/AF-6) Domain Family Member 1 (RASSF1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Ras Association Domain Containing Protein 1 (RASSF1). No significant cross-reactivity or interference between Ras Association Domain Containing Protein 1 (RASSF1) and analogues was observed.

Sensitivity: 0.059 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ras Association Domain Containing Protein 1 (RASSF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ras Association Domain Containing Protein 1 (RASSF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ras Association Domain Containing Protein 1 (RASSF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ras Association Domain Containing Protein 1 (RASSF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ras Association Domain Containing Protein 1 (RASSF1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ras Association Domain Containing Protein 1 (RASSF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for RASSF1

Target: RASSF1 (Ras Association (RalGDS/AF-6) Domain Family Member 1 (RASSF1))

Alternative Name: Ras Association Domain Containing Protein 1 (RASSF1 Products)

Synonyms: RASSF1 ELISA Kit, zgc:92505 ELISA Kit, wu:fc17f02 ELISA Kit, zgc:112224 ELISA Kit, 123F2 ELISA Kit, NORE2A ELISA Kit, RASSF1A ELISA Kit, RDA32 ELISA Kit, REH3P21 ELISA Kit, AA536941 ELISA Kit, AU044980 ELISA Kit, Rassf1A ELISA Kit, Rassf1B ELISA Kit, Rassf1C ELISA Kit, NPR2-like, GATOR1 complex subunit ELISA Kit, Ras association (RalGDS/AF-6) domain family 1 ELISA Kit, Ras association domain family member 1 ELISA Kit, putative rassf1 ELISA Kit, Ras association domain family member 1 L homeolog ELISA Kit, Ras association (RalGDS/AF-6) domain family member 1 ELISA Kit, NPRL2 ELISA Kit, rassf1 ELISA Kit, RASSF1 ELISA Kit, Smp_157340 ELISA Kit, rassf1.L ELISA Kit, Rassf1 ELISA Kit

Background:

Gene Name: Ras Association Domain Containing Protein 1

Gene Aliases: 123F2, NORE2A, RASSF1A, RDA32, REH3P21