RORA ELISA Kit

Catalog No. ABIN5658351

Product Details RORA ELISA Kit

Target: RORA (RAR-Related Orphan Receptor A (RORA))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of RAR Related Orphan Receptor Alpha (RORa). No significant cross-reactivity or interference between RAR Related Orphan Receptor Alpha (RORa) and analogues was observed.

Sensitivity: 0.056 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAR Related Orphan Receptor Alpha (RORa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RAR Related Orphan Receptor Alpha (RORa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RAR Related Orphan Receptor Alpha (RORa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RAR Related Orphan Receptor Alpha (RORa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RAR Related Orphan Receptor Alpha (RORa) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RAR Related Orphan Receptor Alpha (RORa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for RORA

Target: RORA (RAR-Related Orphan Receptor A (RORA))

Alternative Name: RAR Related Orphan Receptor Alpha (RORA Products)

Synonyms: ror1 ELISA Kit, ror2 ELISA Kit, ror3 ELISA Kit, rzra ELISA Kit, nr1f1 ELISA Kit, MGC146531 ELISA Kit, NR1F1 ELISA Kit, ROR1 ELISA Kit, ROR2 ELISA Kit, ROR3 ELISA Kit, RZR-ALPHA ELISA Kit, RZRA ELISA Kit, RORalpha-B ELISA Kit, gb:dq017624 ELISA Kit, rora2 ELISA Kit, 9530021D13Rik ELISA Kit, Nr1f1 ELISA Kit, nmf267 ELISA Kit, sg ELISA Kit, staggerer ELISA Kit, tmgc26 ELISA Kit, RORalpha1 ELISA Kit, RAR related orphan receptor A ELISA Kit, RAR-related orphan receptor A ELISA Kit, RAR-related orphan receptor A, paralog a ELISA Kit, RAR-related orphan receptor alpha ELISA Kit, RORA ELISA Kit, rora ELISA Kit, Rora ELISA Kit, roraa ELISA Kit

Background:

Gene Name: RAR Related Orphan Receptor Alpha

Gene Aliases: ROR1, ROR2, NR1F1, ROR3, RZRA, Nuclear receptor subfamily 1 group F member 1, Retinoid-related orphan receptor-alpha

Gene ID: 6095

UniProt: P35398

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha