RAB5 ELISA Kit

Catalog No. ABIN5658343

Product Details RAB5 ELISA Kit

Target: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of RAB5A, Member RAS Oncogene Family (RAB5A). No significant cross-reactivity or interference between RAB5A, Member RAS Oncogene Family (RAB5A) and analogues was observed.

Sensitivity: 0.056 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAB5A, Member RAS Oncogene Family (RAB5A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RAB5A, Member RAS Oncogene Family (RAB5A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RAB5A, Member RAS Oncogene Family (RAB5A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RAB5A, Member RAS Oncogene Family (RAB5A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RAB5A, Member RAS Oncogene Family (RAB5A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RAB5A, Member RAS Oncogene Family (RAB5A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for RAB5

Target: RAB5 (RAB5A) (RAB5A, Member RAS Oncogene Family (RAB5A))

Alternative Name: RAB5A, Member RAS Oncogene Family (RAB5A Products)

Synonyms: RAB5 ELISA Kit, 2410015H04Rik ELISA Kit, AI663973 ELISA Kit, AU021172 ELISA Kit, nnyRab5a ELISA Kit, DDBDRAFT_0168594 ELISA Kit, DDBDRAFT_0229401 ELISA Kit, DDB_0168594 ELISA Kit, DDB_0229401 ELISA Kit, rab5 ELISA Kit, RAB5A ELISA Kit, Rab5a ELISA Kit, rab5al ELISA Kit, zgc:56644 ELISA Kit, fj38g08 ELISA Kit, hm:zehn1144 ELISA Kit, rab5a ELISA Kit, wu:fj38g08 ELISA Kit, RAB5A, member RAS oncogene family ELISA Kit, rab5A protein ELISA Kit, Rab5a, GTPase ELISA Kit, predicted protein ELISA Kit, Rab GTPase ELISA Kit, RAB5A, member RAS oncogene family L homeolog ELISA Kit, RAB5A, member RAS oncogene family, b ELISA Kit, RAB5A, member RAS oncogene family, a ELISA Kit, RAB5A ELISA Kit, Rab5a ELISA Kit, rab5A ELISA Kit, rab5a ELISA Kit, rab5a.L ELISA Kit, rab5ab ELISA Kit, rab5aa ELISA Kit

Background:

Gene Name: RAB5A, Member RAS Oncogene Family

Gene Aliases: RAB5

Pathways: Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity