QDPR ELISA Kit

Catalog No. ABIN5658327

Product Details QDPR ELISA Kit

Target: QDPR (Quinoid Dihydropteridine Reductase (QDPR))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Quinoid Dihydropteridine Reductase (QDPR). No significant cross-reactivity or interference between Quinoid Dihydropteridine Reductase (QDPR) and analogues was observed.

Sensitivity: 0.051 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Quinoid Dihydropteridine Reductase (QDPR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Quinoid Dihydropteridine Reductase (QDPR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Quinoid Dihydropteridine Reductase (QDPR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Quinoid Dihydropteridine Reductase (QDPR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Quinoid Dihydropteridine Reductase (QDPR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Quinoid Dihydropteridine Reductase (QDPR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for QDPR

Target: QDPR (Quinoid Dihydropteridine Reductase (QDPR))

Alternative Name: Quinoid Dihydropteridine Reductase (QDPR Products)

Synonyms: DHPR ELISA Kit, PKU2 ELISA Kit, SDR33C1 ELISA Kit, 2610008L04Rik ELISA Kit, D5Ertd371e ELISA Kit, Dhpr ELISA Kit, QDPR ELISA Kit, qdpr ELISA Kit, MGC131372 ELISA Kit, DDBDRAFT_0168923 ELISA Kit, DDBDRAFT_0237752 ELISA Kit, DDB_0168923 ELISA Kit, DDB_0237752 ELISA Kit, CG4665 ELISA Kit, Dmel\\CG4665 ELISA Kit, si:ch211-89f22.2 ELISA Kit, quinoid dihydropteridine reductase ELISA Kit, quinoid dihydropteridine reductase S homeolog ELISA Kit, dihydropteridine reductase ELISA Kit, Dihydropteridine reductase ELISA Kit, bifunctional oxygen-insensitive NAD(P)H nitroreductase/dihydropteridine reductase NfnB ELISA Kit, quinoid dihydropteridine reductase a ELISA Kit, QDPR ELISA Kit, Qdpr ELISA Kit, qdpr ELISA Kit, qdpr.S ELISA Kit, Dhpr ELISA Kit, nfnB ELISA Kit, LOC5568428 ELISA Kit, dhpr ELISA Kit, qdpra ELISA Kit

Background:

Gene Name: Quinoid Dihydropteridine Reductase

Gene Aliases: DHPR, PKU2, SDR33C1, HDHPR, 6,7-Dihydropteridine Reductase, Short Chain Dehydrogenase/Reductase Family 33C,Member 1

Gene ID: 5860

UniProt: P09417