QSOX1 ELISA Kit

Catalog No. ABIN5658325

Product Details QSOX1 ELISA Kit

Target: QSOX1 (Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1). No significant cross-reactivity or interference between Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1) and analogues was observed.

Sensitivity: 0.131 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for QSOX1

Target: QSOX1 (Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1))

Alternative Name: Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1 Products)

Synonyms: qscn6l1 ELISA Kit, Qscn6 ELISA Kit, Qsox ELISA Kit, Sox-2 ELISA Kit, rSOx ELISA Kit, Q6 ELISA Kit, QSCN6 ELISA Kit, 1300003H02Rik ELISA Kit, QSOX ELISA Kit, SOx ELISA Kit, GEC-3 ELISA Kit, GEC3 ELISA Kit, SOX3 ELISA Kit, ATQSOX1 ELISA Kit, QSO2 ELISA Kit, T15D22.7 ELISA Kit, T15D22_7 ELISA Kit, quiescin-sulfhydryl oxidase 1 ELISA Kit, quiescin sulfhydryl oxidase 1 ELISA Kit, quiescin sulfhydryl oxidase 1 L homeolog ELISA Kit, quiescin Q6 sulfhydryl oxidase 1 ELISA Kit, quiescin-sulfhydryl oxidase 1 ELISA Kit, QSOX1 ELISA Kit, qsox1 ELISA Kit, qsox1.L ELISA Kit, Qsox1 ELISA Kit

Background:

Gene Name: Quiescin Q6 Sulfhydryl Oxidase 1

Gene Aliases: Q6, QSCN6

Gene ID: 5768

UniProt: O00391

Pathways: Cell RedoxHomeostasis