PKM ELISA Kit

Catalog No. ABIN5658324

Product Details PKM ELISA Kit

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.94 ng/mL - 60 ng/mL

Minimum Detection Limit: 0.94 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Pyruvate Kinase, Muscle (PKM2). No significant cross-reactivity or interference between Pyruvate Kinase, Muscle (PKM2) and analogues was observed.

Sensitivity: 0.42 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Kinase, Muscle (PKM2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Kinase, Muscle (PKM2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Kinase, Muscle (PKM2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pyruvate Kinase, Muscle (PKM2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pyruvate Kinase, Muscle (PKM2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pyruvate Kinase, Muscle (PKM2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for PKM

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Alternative Name: Pyruvate Kinase, Muscle (PKM Products)

Synonyms: CTHBP ELISA Kit, OIP3 ELISA Kit, PK3 ELISA Kit, PKM2 ELISA Kit, TCB ELISA Kit, THBP1 ELISA Kit, AA414905 ELISA Kit, AL024370 ELISA Kit, AL024424 ELISA Kit, Pk-2 ELISA Kit, Pk-3 ELISA Kit, Pk3 ELISA Kit, Pkm2 ELISA Kit, PKM12 ELISA Kit, CG7070 ELISA Kit, Dmel\\CG7070 ELISA Kit, PYK ELISA Kit, Pyk ELISA Kit, cb1029 ELISA Kit, fj33f03 ELISA Kit, pkm2 ELISA Kit, pkm2a ELISA Kit, wu:fj33f03 ELISA Kit, MGC53388 ELISA Kit, PKM ELISA Kit, PK-S ELISA Kit, pk3 ELISA Kit, pkm2b ELISA Kit, zgc:92037 ELISA Kit, pyruvate kinase M1/2 ELISA Kit, pyruvate kinase, muscle ELISA Kit, Pyruvate kinase ELISA Kit, pyruvate kinase M1/2a ELISA Kit, pyruvate kinase PKM ELISA Kit, pyruvate kinase (PK) ELISA Kit, pyruvate kinase ELISA Kit, pyruvate kinase M1/2 S homeolog ELISA Kit, pyruvate kinase M1/2b ELISA Kit, PKM ELISA Kit, Pkm ELISA Kit, PyK ELISA Kit, pkma ELISA Kit, pkm ELISA Kit, LOC710798 ELISA Kit, pyk ELISA Kit, pk ELISA Kit, LOC100534543 ELISA Kit, pkm.S ELISA Kit, pkmb ELISA Kit

Background:

Gene Name: Pyruvate Kinase, Muscle

Gene Aliases: M2PK, PKM, M2-PK, PKM1, CTHBP, OIP3, PK3, PKM, TCB, THBP1, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, Cytosolic thyroid hormone-binding protein

Gene ID: 25630

UniProt: P11980

Pathways: Warburg Effect