PDHB ELISA Kit

Catalog No. ABIN5658314

Product Details PDHB ELISA Kit

Target: PDHB (Pyruvate Dehydrogenase beta (PDHB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Pyruvate Dehydrogenase Beta (PDHb). No significant cross-reactivity or interference between Pyruvate Dehydrogenase Beta (PDHb) and analogues was observed.

Sensitivity: 0.65 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Dehydrogenase Beta (PDHb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Dehydrogenase Beta (PDHb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Dehydrogenase Beta (PDHb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pyruvate Dehydrogenase Beta (PDHb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pyruvate Dehydrogenase Beta (PDHb) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pyruvate Dehydrogenase Beta (PDHb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for PDHB

Target: PDHB (Pyruvate Dehydrogenase beta (PDHB))

Alternative Name: Pyruvate Dehydrogenase Beta (PDHB Products)

Synonyms: CG 11876 ELISA Kit, Dmel\\CG11876 ELISA Kit, E1beta ELISA Kit, PDHB ELISA Kit, zgc:64062 ELISA Kit, wu:fc76a05 ELISA Kit, PDHBD ELISA Kit, PDHE1-B ELISA Kit, PHE1B ELISA Kit, 2610103L06Rik ELISA Kit, AL024199 ELISA Kit, C81408 ELISA Kit, CG11876 gene product from transcript CG11876-RA ELISA Kit, pyruvate dehydrogenase E1 beta subunit ELISA Kit, pyruvate dehydrogenase (lipoamide) beta ELISA Kit, CG11876 ELISA Kit, pdhb ELISA Kit, PDHB ELISA Kit, Pdhb ELISA Kit

Background:

Gene Name: Pyruvate Dehydrogenase Beta

Gene Aliases: PDH-B, PHE1B, PHE1-B, Pyruvate Dehydrogenase(lipoamide)Beta

Gene ID: 5162

UniProt: P11177

Pathways: Warburg Effect