PC ELISA Kit

Catalog No. ABIN5658309

Product Details PC ELISA Kit

Target: PC (Pyruvate Carboxylase (PC))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Pyruvate Carboxylase (PC). No significant cross-reactivity or interference between Pyruvate Carboxylase (PC) and analogues was observed.

Sensitivity: 0.63 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Carboxylase (PC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Carboxylase (PC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Carboxylase (PC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pyruvate Carboxylase (PC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pyruvate Carboxylase (PC) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pyruvate Carboxylase (PC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for PC

Target: PC (Pyruvate Carboxylase (PC))

Alternative Name: Pyruvate Carboxylase (PC Products)

Synonyms: BcDNA ELISA Kit, BcDNA:GH06348 ELISA Kit, Dmel\\CG1516 ELISA Kit, PC ELISA Kit, pyc ELISA Kit, wu:fb82g08 ELISA Kit, wu:fj85g01 ELISA Kit, PCX ELISA Kit, BA4157 ELISA Kit, Pc ELISA Kit, Pcb ELISA Kit, PCB ELISA Kit, Pcx ELISA Kit, Pyruvate carboxylase ELISA Kit, pyruvate carboxylase b ELISA Kit, PYruvate Carboxylase ELISA Kit, pyruvate carboxylase ELISA Kit, pyruvate carboxylase Pyr1 ELISA Kit, PCB ELISA Kit, pcxb ELISA Kit, pyc-1 ELISA Kit, PC ELISA Kit, pyc ELISA Kit, pyr1 ELISA Kit, CNF00650 ELISA Kit, PYC1 ELISA Kit, Ndas_1152 ELISA Kit, Ilyop_2503 ELISA Kit, Calni_0495 ELISA Kit, Ftrac_1004 ELISA Kit, Plabr_3574 ELISA Kit, Sgly_2936 ELISA Kit, Celly_0558 ELISA Kit, Hipma_0131 ELISA Kit, Halhy_1567 ELISA Kit, Mesop_1269 ELISA Kit, Pcx ELISA Kit, Pc ELISA Kit

Background:

Gene Name: Pyruvate Carboxylase

Gene Aliases: PCB

Gene ID: 5091

UniProt: P11498