PPOX ELISA Kit

Catalog No. ABIN5658296

Product Details PPOX ELISA Kit

Target: PPOX (Protoporphyrinogen Oxidase (PPOX))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Protoporphyrinogen Oxidase (PPOX). No significant cross-reactivity or interference between Protoporphyrinogen Oxidase (PPOX) and analogues was observed.

Sensitivity: 0.053 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protoporphyrinogen Oxidase (PPOX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protoporphyrinogen Oxidase (PPOX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protoporphyrinogen Oxidase (PPOX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protoporphyrinogen Oxidase (PPOX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protoporphyrinogen Oxidase (PPOX) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protoporphyrinogen Oxidase (PPOX) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for PPOX

Target: PPOX (Protoporphyrinogen Oxidase (PPOX))

Alternative Name: Protoporphyrinogen Oxidase (PPOX Products)

Synonyms: PPO ELISA Kit, V290M ELISA Kit, VP ELISA Kit, Ppo ELISA Kit, BcDNA:LD41392 ELISA Kit, CG5796 ELISA Kit, Dmel\\CG5796 ELISA Kit, ESTS:9F2T ELISA Kit, zgc:123252 ELISA Kit, HEMG1 ELISA Kit, PPO1 ELISA Kit, PROTOPORPHYRINOGEN OXIDASE ELISA Kit, TC0121 ELISA Kit, hemG ELISA Kit, protoporphyrinogen oxidase ELISA Kit, Protoporphyrinogen oxidase ELISA Kit, Flavin containing amine oxidoreductase family ELISA Kit, polyphenol oxidase, chloroplastic ELISA Kit, PPOX ELISA Kit, Ppox ELISA Kit, ppox ELISA Kit, hemY ELISA Kit, TC_RS00645 ELISA Kit, Mrub_1406 ELISA Kit, Mesil_2992 ELISA Kit, Trad_0583 ELISA Kit, Calni_0746 ELISA Kit, Ocepr_1049 ELISA Kit, Tmar_0766 ELISA Kit, Intca_1907 ELISA Kit, Despr_0642 ELISA Kit, Plabr_2407 ELISA Kit, Deipr_2130 ELISA Kit, Hipma_0622 ELISA Kit, LOC110782963 ELISA Kit

Background:

Gene Name: Protoporphyrinogen Oxidase

Gene Aliases: VP, PPO, V290M, Variegate Porphyria

Gene ID: 5498

UniProt: P50336

Pathways: Synaptic Membrane, Feeding Behaviour