PCDH15 ELISA Kit

Catalog No. ABIN5658291

Product Details PCDH15 ELISA Kit

Target: PCDH15 (Protocadherin-15 (PCDH15))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Protocadherin 15 (PCDH15). No significant cross-reactivity or interference between Protocadherin 15 (PCDH15) and analogues was observed.

Sensitivity: 0.065 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protocadherin 15 (PCDH15). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protocadherin 15 (PCDH15). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protocadherin 15 (PCDH15), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protocadherin 15 (PCDH15) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protocadherin 15 (PCDH15) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protocadherin 15 (PCDH15) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for PCDH15

Target: PCDH15 (Protocadherin-15 (PCDH15))

Alternative Name: Protocadherin 15 (PCDH15 Products)

Synonyms: CDHR15 ELISA Kit, DFNB23 ELISA Kit, USH1F ELISA Kit, BB078305 ELISA Kit, ENSMUSG00000046980 ELISA Kit, Gm9815 ELISA Kit, Ush1f ELISA Kit, av ELISA Kit, nmf19 ELISA Kit, protocadherin-15 ELISA Kit, protocadherin related 15 ELISA Kit, protocadherin-15 ELISA Kit, protocadherin 15 ELISA Kit, PCDH15 ELISA Kit, CpipJ_CPIJ005081 ELISA Kit, Pcdh15 ELISA Kit

Background:

Gene Name: Protocadherin 15

Gene Aliases: USH1F, DFNB23, CDHR15, Deafness,Autosomal Recessive 23, Cadherin-Related Family Member 15

Gene ID: 11994

UniProt: Q99PJ1

Pathways: Sensory Perception of Sound