PPM1A ELISA Kit
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- Target See all PPM1A ELISA Kits
- PPM1A (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1A (PPM1A))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.312 ng/mL - 20 ng/mL
- Minimum Detection Limit
- 0.312 ng/mL
- Application
- ELISA
- Sample Type
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A). No significant cross-reactivity or interference between Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) and analogues was observed.
- Sensitivity
- 0.123 ng/mL
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- Comment
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Assay Time
- 3 h
- Plate
- Pre-coated
- Protocol
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Assay Precision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Handling Advice
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Expiry Date
- 4-8 months
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- Target See all PPM1A ELISA Kits
- PPM1A (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1A (PPM1A))
- Alternative Name
- Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A Products)
- Synonyms
- PP2C-ALPHA ELISA Kit, PP2CA ELISA Kit, PP2Calpha ELISA Kit, pp2ca1 ELISA Kit, ppp1r13b ELISA Kit, si:ch211-253p18.1 ELISA Kit, wu:fd42g12 ELISA Kit, zgc:153820 ELISA Kit, 2310003C21Rik ELISA Kit, 2900017D14Rik ELISA Kit, AI427932 ELISA Kit, AU017636 ELISA Kit, MMPa-2 ELISA Kit, MPPa-1 ELISA Kit, Pp2c1 ELISA Kit, pp2c-alpha ELISA Kit, pp2ca ELISA Kit, pp2calpha ELISA Kit, pp2ca2 ELISA Kit, protein phosphatase, Mg2+/Mn2+ dependent 1A ELISA Kit, protein phosphatase, Mg2+/Mn2+ dependent, 1Ab ELISA Kit, protein phosphatase 1A, magnesium dependent, alpha isoform ELISA Kit, protein phosphatase, Mg2+/Mn2+ dependent, 1A ELISA Kit, protein phosphatase, Mg2+/Mn2+ dependent 1A L homeolog ELISA Kit, protein phosphatase, Mg2+/Mn2+ dependent, 1Aa ELISA Kit, PPM1A ELISA Kit, ppm1ab ELISA Kit, Ppm1a ELISA Kit, ppm1a.L ELISA Kit, ppm1aa ELISA Kit
- Background
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Gene Name: Protein Phosphatase, Mg2+/Mn2+ Dependent 1A
Gene Aliases: PP2C-ALPHA, PP2CA, Phosphatase 2C Alpha, Protein Phosphatase 1A(Formerly 2C)Magnesium-Dependent,Alpha
- Gene ID
- 5494
- UniProt
- P35813
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