PRKAB1 ELISA Kit

Catalog No. ABIN5658232

Product Details PRKAB1 ELISA Kit

Target: PRKAB1 (Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Protein Kinase, AMP Activated Beta 1 (PRKAb1). No significant cross-reactivity or interference between Protein Kinase, AMP Activated Beta 1 (PRKAb1) and analogues was observed.

Sensitivity: 0.124 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase, AMP Activated Beta 1 (PRKAb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase, AMP Activated Beta 1 (PRKAb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase, AMP Activated Beta 1 (PRKAb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase, AMP Activated Beta 1 (PRKAb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Kinase, AMP Activated Beta 1 (PRKAb1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Kinase, AMP Activated Beta 1 (PRKAb1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for PRKAB1

Target: PRKAB1 (Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1))

Alternative Name: Protein Kinase, AMP Activated Beta 1 (PRKAB1 Products)

Synonyms: AMPK ELISA Kit, HAMPKb ELISA Kit, 1300015D22Rik ELISA Kit, AU021155 ELISA Kit, E430008F22 ELISA Kit, MGC82489 ELISA Kit, prkab1 ELISA Kit, wu:fk93d05 ELISA Kit, wu:fw87e09 ELISA Kit, zgc:56652 ELISA Kit, zgc:76975 ELISA Kit, zgc:92228 ELISA Kit, protein kinase AMP-activated non-catalytic subunit beta 1 ELISA Kit, protein kinase, AMP-activated, beta 1 non-catalytic subunit ELISA Kit, protein kinase, AMP-activated, beta 1 non-catalytic subunit S homeolog ELISA Kit, protein kinase, AMP-activated, beta 1 non-catalytic subunit, b ELISA Kit, protein kinase, AMP-activated, beta 1 non-catalytic subunit, a ELISA Kit, PRKAB1 ELISA Kit, Prkab1 ELISA Kit, prkab1.S ELISA Kit, prkab1 ELISA Kit, prkab1b ELISA Kit, prkab1a ELISA Kit

Background:

Gene Name: Protein Kinase, AMP Activated Beta 1

Gene Aliases: AMPK, HAMPKb, 5'-AMP-Activated Protein Kinase Subunit Beta-1 Non-Catalytic Subunit, Adenosine Monophosphate Activated Protein Kinase(AMPK)

Gene ID: 5564

UniProt: Q9Y478

Pathways: AMPK Signaling, Warburg Effect