PDIA4 ELISA Kit

Catalog No. ABIN5658202

Product Details PDIA4 ELISA Kit

Target: PDIA4 (Protein Disulfide Isomerase Family A, Member 4 (PDIA4))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Protein Disulfide Isomerase A4 (PDIA4). No significant cross-reactivity or interference between Protein Disulfide Isomerase A4 (PDIA4) and analogues was observed.

Sensitivity: 0.65 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Disulfide Isomerase A4 (PDIA4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Disulfide Isomerase A4 (PDIA4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Disulfide Isomerase A4 (PDIA4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Disulfide Isomerase A4 (PDIA4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Disulfide Isomerase A4 (PDIA4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Disulfide Isomerase A4 (PDIA4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for PDIA4

Target: PDIA4 (Protein Disulfide Isomerase Family A, Member 4 (PDIA4))

Alternative Name: Protein Disulfide Isomerase A4 (PDIA4 Products)

Synonyms: LOC100229535 ELISA Kit, cai ELISA Kit, wu:fd20c07 ELISA Kit, zgc:113965 ELISA Kit, zgc:136625 ELISA Kit, zgc:56014 ELISA Kit, zgc:77773 ELISA Kit, AI987846 ELISA Kit, Cai ELISA Kit, ERp-72 ELISA Kit, Erp72 ELISA Kit, ERP70 ELISA Kit, ERP72 ELISA Kit, Erp70 ELISA Kit, protein disulfide isomerase family A member 4 ELISA Kit, protein disulfide isomerase family A, member 4 ELISA Kit, protein disulfide isomerase associated 4 ELISA Kit, protein disulfide isomerase family A member 4 L homeolog ELISA Kit, PDIA4 ELISA Kit, pdia4 ELISA Kit, Pdia4 ELISA Kit, pdia4.L ELISA Kit

Background:

Gene Name: Protein Disulfide Isomerase A4

Gene Aliases: PDI-A4, ERP70, ERP72, Protein Disulfide Isomerase-Associated 4, Endoplasmic reticulum resident protein 70

Gene ID: 9601

UniProt: P13667

Pathways: Thyroid Hormone Synthesis, Cell RedoxHomeostasis