PA2G4 ELISA Kit

Catalog No. ABIN5658111

Product Details PA2G4 ELISA Kit

Target: PA2G4 (Proliferation-Associated 2G4, 38kDa (PA2G4))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Proliferation Associated Protein 2G4 (PA2G4). No significant cross-reactivity or interference between Proliferation Associated Protein 2G4 (PA2G4) and analogues was observed.

Sensitivity: 0.114 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proliferation Associated Protein 2G4 (PA2G4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proliferation Associated Protein 2G4 (PA2G4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proliferation Associated Protein 2G4 (PA2G4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Proliferation Associated Protein 2G4 (PA2G4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Proliferation Associated Protein 2G4 (PA2G4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Proliferation Associated Protein 2G4 (PA2G4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for PA2G4

Target: PA2G4 (Proliferation-Associated 2G4, 38kDa (PA2G4))

Alternative Name: Proliferation Associated Protein 2G4 (PA2G4 Products)

Synonyms: EBP1 ELISA Kit, HG4-1 ELISA Kit, p38-2G4 ELISA Kit, Ebp1 ELISA Kit, pa2g4 ELISA Kit, si:dz150i12.2 ELISA Kit, wu:fb19b11 ELISA Kit, wu:ft56d05 ELISA Kit, zgc:86732 ELISA Kit, ebp1 ELISA Kit, hg4-1 ELISA Kit, p38-2g4 ELISA Kit, pa2g4-a ELISA Kit, PA2G4 ELISA Kit, DKFZp469G2132 ELISA Kit, Pa2g4 ELISA Kit, MGC81621 ELISA Kit, 38kDa ELISA Kit, AA672939 ELISA Kit, Plfap ELISA Kit, proliferation-associated 2G4 ELISA Kit, proliferation-associated 2G4, a ELISA Kit, proliferation-associated 2G4, 38kDa ELISA Kit, proliferation-associated 2G4 L homeolog ELISA Kit, proliferation-associated protein 2G4 ELISA Kit, proliferation-associated 2G4 S homeolog ELISA Kit, proliferation-associated protein 2G4 pseudogene ELISA Kit, PA2G4 ELISA Kit, Pa2g4 ELISA Kit, pa2g4a ELISA Kit, pa2g4.L ELISA Kit, pa2g4 ELISA Kit, LOC100519773 ELISA Kit, pa2g4.S ELISA Kit, LOC693641 ELISA Kit

Background:

Gene Name: Proliferation Associated Protein 2G4

Gene Aliases: EBP1, HG4-1, p38-2G4, Cell cycle protein p38-2G4 homolog, ErbB3-binding protein 1

Gene ID: 5036

UniProt: Q9UQ80

Pathways: Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Toll-Like Receptors Cascades