PDCD6 ELISA Kit

Catalog No. ABIN5658083

Product Details PDCD6 ELISA Kit

Target: PDCD6 (Programmed Cell Death 6 (PDCD6))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Programmed Cell Death Protein 6 (PDCD6). No significant cross-reactivity or interference between Programmed Cell Death Protein 6 (PDCD6) and analogues was observed.

Sensitivity: 0.118 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Programmed Cell Death Protein 6 (PDCD6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Programmed Cell Death Protein 6 (PDCD6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Programmed Cell Death Protein 6 (PDCD6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Programmed Cell Death Protein 6 (PDCD6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Programmed Cell Death Protein 6 (PDCD6) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Programmed Cell Death Protein 6 (PDCD6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for PDCD6

Target: PDCD6 (Programmed Cell Death 6 (PDCD6))

Alternative Name: Programmed Cell Death Protein 6 (PDCD6 Products)

Synonyms: pdcd6 ELISA Kit, MGC52821 ELISA Kit, MGC132114 ELISA Kit, zgc:63952 ELISA Kit, PDCD6 ELISA Kit, ALG-2 ELISA Kit, PEF1B ELISA Kit, AV299538 ELISA Kit, Alg2 ELISA Kit, MA-3 ELISA Kit, PS2 ELISA Kit, alg-2 ELISA Kit, programmed cell death 6 L homeolog ELISA Kit, programmed cell death 6 ELISA Kit, pdcd6.L ELISA Kit, pdcd6 ELISA Kit, PDCD6 ELISA Kit, Pdcd6 ELISA Kit

Background:

Gene Name: Programmed Cell Death Protein 6

Gene Aliases: ALG-2, PEF1B, Apoptosis-Linked Gene-2, Probable calcium-binding protein ALG-2

Gene ID: 10016

UniProt: O75340

Pathways: Positive Regulation of Endopeptidase Activity