PLOD1 ELISA Kit

Catalog No. ABIN5658057

Product Details PLOD1 ELISA Kit

Target: PLOD1 (Procollagen-Lysine,2-Oxoglutarate 5-Dioxygenase 1 (PLOD1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Procollagen Lysine-1,2-Oxoglutarate-5-Dioxygenase 1 (PLOD1). No significant cross-reactivity or interference between Procollagen Lysine-1,2-Oxoglutarate-5-Dioxygenase 1 (PLOD1) and analogues was observed.

Sensitivity: 0.066 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Procollagen Lysine-1,2-Oxoglutarate-5-Dioxygenase 1 (PLOD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Procollagen Lysine-1,2-Oxoglutarate-5-Dioxygenase 1 (PLOD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Procollagen Lysine-1,2-Oxoglutarate-5-Dioxygenase 1 (PLOD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Procollagen Lysine-1,2-Oxoglutarate-5-Dioxygenase 1 (PLOD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Procollagen Lysine-1,2-Oxoglutarate-5-Dioxygenase 1 (PLOD1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Procollagen Lysine-1,2-Oxoglutarate-5-Dioxygenase 1 (PLOD1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for PLOD1

Target: PLOD1 (Procollagen-Lysine,2-Oxoglutarate 5-Dioxygenase 1 (PLOD1))

Alternative Name: Procollagen Lysine-1,2-Oxoglutarate-5-Dioxygenase 1 (PLOD1 Products)

Synonyms: EDS6 ELISA Kit, LH ELISA Kit, LH1 ELISA Kit, LLH ELISA Kit, PLOD ELISA Kit, Plod ELISA Kit, procollagen-lysine ELISA Kit, 2410042F05Rik ELISA Kit, AI854890 ELISA Kit, AV007929 ELISA Kit, Lh1 ELISA Kit, plod1 ELISA Kit, ik:tdsubs_rzpd609-29b19 ELISA Kit, plod ELISA Kit, wu:fc29c10 ELISA Kit, wu:fc95g08 ELISA Kit, wu:fi18a05 ELISA Kit, zgc:152876 ELISA Kit, procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 ELISA Kit, procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 ELISA Kit, procollagen-lysine,2-oxoglutarate 5-dioxygenase 3 ELISA Kit, procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 L homeolog ELISA Kit, procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1a ELISA Kit, PLOD1 ELISA Kit, Plod1 ELISA Kit, PLOD3 ELISA Kit, plod1.L ELISA Kit, plod1a ELISA Kit

Background:

Gene Name: Procollagen Lysine-1,2-Oxoglutarate-5-Dioxygenase 1

Gene Aliases: LLH, PLOD, LH1, Lysine Hydroxylase,Ehlers-Danlos Syndrome Type VI, Lysyl hydroxylase 1

Gene ID: 5351

UniProt: Q02809