PRNP ELISA Kit

Catalog No. ABIN5658014

Product Details PRNP ELISA Kit

Target: PRNP (Prion Protein (PRNP))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Prion Protein (PRNP). No significant cross-reactivity or interference between Prion Protein (PRNP) and analogues was observed.

Sensitivity: 0.055 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prion Protein (PRNP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prion Protein (PRNP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prion Protein (PRNP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prion Protein (PRNP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prion Protein (PRNP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prion Protein (PRNP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for PRNP

Target: PRNP (Prion Protein (PRNP))

Alternative Name: Prion Protein (PRNP Products)

Synonyms: ASCR ELISA Kit, AltPrP ELISA Kit, CD230 ELISA Kit, CJD ELISA Kit, GSS ELISA Kit, KURU ELISA Kit, PRIP ELISA Kit, PrP ELISA Kit, PrP27-30 ELISA Kit, PrP33-35C ELISA Kit, PrPc ELISA Kit, p27-30 ELISA Kit, Prn ELISA Kit, Prn-p ELISA Kit, prp ELISA Kit, cjd ELISA Kit, prion ELISA Kit, prp(c) ELISA Kit, MGC84114 ELISA Kit, PRP ELISA Kit, PRPC ELISA Kit, Prp ELISA Kit, SIP ELISA Kit, PRNP ELISA Kit, AA960666 ELISA Kit, AI325101 ELISA Kit, PrP ELISA Kit, PrPC ELISA Kit, PrPSc ELISA Kit, Prn-i ELISA Kit, Sinc ELISA Kit, prP27-30 ELISA Kit, prP33-35C ELISA Kit, PrP-1 ELISA Kit, PrP1 ELISA Kit, prnprs1 ELISA Kit, stPrP-1 ELISA Kit, zgc:123004 ELISA Kit, prion protein ELISA Kit, prion protein S homeolog ELISA Kit, prion protein b ELISA Kit, PRNP ELISA Kit, Prnp ELISA Kit, prnp.S ELISA Kit, prnpb ELISA Kit

Background:

Gene Name: Prion Protein

Gene Aliases: CD230, GSS, ASCR, CJD, PRIP, PrP, PrP2730, PrPc, Major prion protein, Creutzfeldt-Jakob Disease, Gerstmann-Strausler-Scheinker Syndrome, Fatal Familial Insomnia

Gene ID: 5621

UniProt: P04156

Pathways: Transition Metal Ion Homeostasis, Activated T Cell Proliferation