KCNJ10 ELISA Kit

Catalog No. ABIN5657988

Product Details KCNJ10 ELISA Kit

Target: KCNJ10 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 10 (KCNJ10))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Potassium Inwardly Rectifying Channel Subfamily J, Member 10 (KCNJ10). No significant cross-reactivity or interference between Potassium Inwardly Rectifying Channel Subfamily J, Member 10 (KCNJ10) and analogues was observed.

Sensitivity: 0.58 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Potassium Inwardly Rectifying Channel Subfamily J, Member 10 (KCNJ10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Potassium Inwardly Rectifying Channel Subfamily J, Member 10 (KCNJ10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Potassium Inwardly Rectifying Channel Subfamily J, Member 10 (KCNJ10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Potassium Inwardly Rectifying Channel Subfamily J, Member 10 (KCNJ10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Potassium Inwardly Rectifying Channel Subfamily J, Member 10 (KCNJ10) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Potassium Inwardly Rectifying Channel Subfamily J, Member 10 (KCNJ10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for KCNJ10

Target: KCNJ10 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 10 (KCNJ10))

Alternative Name: Potassium Inwardly Rectifying Channel Subfamily J, Member 10 (KCNJ10 Products)

Synonyms: kir1.2 ELISA Kit, kir4.1 ELISA Kit, birk-10 ELISA Kit, MGC145926 ELISA Kit, BIR10 ELISA Kit, BIRK-1 ELISA Kit, Kir1.2 ELISA Kit, Kir4.1 ELISA Kit, BIRK-10 ELISA Kit, KCNJ13-PEN ELISA Kit, KIR1.2 ELISA Kit, KIR4.1 ELISA Kit, SESAME ELISA Kit, potassium channel, inwardly rectifying subfamily J, member 10 L homeolog ELISA Kit, potassium voltage-gated channel subfamily J member 10 ELISA Kit, potassium channel, inwardly rectifying subfamily J, member 10 ELISA Kit, potassium inwardly-rectifying channel, subfamily J, member 10 ELISA Kit, kcnj10.L ELISA Kit, KCNJ10 ELISA Kit, kcnj10 ELISA Kit, Kcnj10 ELISA Kit

Background:

Gene Name: Potassium Inwardly Rectifying Channel Subfamily J, Member 10

Gene Aliases: Kir4.1, Kir1.2, ATP-sensitive inward rectifier potassium channel 10, ATP-dependent inwardly rectifying potassium channel Kir4.1, Inward rectifier K(+) channel Kir1.2

Gene ID: 3766

UniProt: P78508

Pathways: Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity