BMI1 ELISA Kit

Catalog No. ABIN5657978

Product Details BMI1 ELISA Kit

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Polycomb Group Ring Finger Protein 4 (PCGF4). No significant cross-reactivity or interference between Polycomb Group Ring Finger Protein 4 (PCGF4) and analogues was observed.

Sensitivity: 0.122 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Polycomb Group Ring Finger Protein 4 (PCGF4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Polycomb Group Ring Finger Protein 4 (PCGF4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Polycomb Group Ring Finger Protein 4 (PCGF4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Polycomb Group Ring Finger Protein 4 (PCGF4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Polycomb Group Ring Finger Protein 4 (PCGF4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Polycomb Group Ring Finger Protein 4 (PCGF4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for BMI1

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Alternative Name: Polycomb Group Ring Finger Protein 4 (BMI1 Products)

Synonyms: FLVI2/BMI1 ELISA Kit, PCGF4 ELISA Kit, RNF51 ELISA Kit, AW546694 ELISA Kit, Bmi-1 ELISA Kit, Pcgf4 ELISA Kit, bmi1 ELISA Kit, pcgf4 ELISA Kit, psc1 ELISA Kit, wu:fb17g03 ELISA Kit, wu:fd18f06 ELISA Kit, BMI-1 ELISA Kit, pcgf4b ELISA Kit, bmi-1 ELISA Kit, bmi1-a ELISA Kit, bmi1-b ELISA Kit, bmi1b ELISA Kit, rnf51 ELISA Kit, xbmi-1 ELISA Kit, BMI1 proto-oncogene, polycomb ring finger ELISA Kit, BMI1 polycomb ring finger oncogene ELISA Kit, Bmi1 polycomb ring finger oncogene ELISA Kit, bmi1 polycomb ring finger oncogene 1a ELISA Kit, bmi1 polycomb ring finger oncogene 1b ELISA Kit, BMI1 proto-oncogene, polycomb ring finger L homeolog ELISA Kit, BMI1 ELISA Kit, LOC100230513 ELISA Kit, Bmi1 ELISA Kit, bmi1a ELISA Kit, bmi1b ELISA Kit, bmi1.L ELISA Kit

Background:

Gene Name: Polycomb Group Ring Finger Protein 4

Gene Aliases: BMI1, RNF51, BMI1 Polycomb Ring Finger Oncogene, RING finger protein 51, Polycomb complex protein BMI-1

Gene ID: 100532731

UniProt: P35226

Pathways: Cell Division Cycle, Autophagy