PDGF-AA Homodimer ELISA Kit

Catalog No. ABIN5657888

Product Details

Target: PDGF-AA Homodimer

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Platelet Derived Growth Factor AA (PDGFAA). No significant cross-reactivity or interference between Platelet Derived Growth Factor AA (PDGFAA) and analogues was observed.

Sensitivity: 0.054 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Derived Growth Factor AA (PDGFAA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Platelet Derived Growth Factor AA (PDGFAA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Platelet Derived Growth Factor AA (PDGFAA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Platelet Derived Growth Factor AA (PDGFAA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Derived Growth Factor AA (PDGFAA) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Derived Growth Factor AA (PDGFAA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: PDGF-AA Homodimer

Alternative Name: Platelet Derived Growth Factor AA

Synonyms: PDGF-A ELISA Kit, PDGF1 ELISA Kit, AA409063 ELISA Kit, CD142 ELISA Kit, Cf-3 ELISA Kit, Cf3 ELISA Kit, TF ELISA Kit, PDGFACP ELISA Kit, platelet derived growth factor subunit A ELISA Kit, coagulation factor III ELISA Kit, platelet derived growth factor, alpha ELISA Kit, PDGFA ELISA Kit, F3 ELISA Kit, Pdgfa ELISA Kit

Background:

Gene Name: Platelet Derived Growth Factor AA

Gene Aliases: PDGF-AA

Gene ID: 5154

UniProt: P04085