Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

PIM1 CLIA Kit

PIM1 Reactivity: Human Chemiluminescent Sandwich ELISA 0.781 ng/mL - 50 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Catalog No. ABIN5657829
  • Target See all PIM1 CLIA Kits
    PIM1 (Pim-1 Oncogene (PIM1))
    Reactivity
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Chemiluminescent
    Method Type
    Sandwich ELISA
    Detection Range
    0.781 ng/mL - 50 ng/mL
    Minimum Detection Limit
    0.781 ng/mL
    Application
    ELISA
    Sample Type
    Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Pim-1 Oncogene (PIM1). No significant cross-reactivity or interference between Pim-1 Oncogene (PIM1) and analogues was observed.
    Sensitivity
    0.03 ng/mL
    Featured
    Discover our best selling PIM1 ELISA Kit
    Top Product
    Discover our top product PIM1 ELISA Kit
  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    2 - 3 h
    Plate
    Pre-coated
    Protocol
    The microplate provided in this kit has been pre-coated with an antibody specific to Pim-1 Oncogene (PIM1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Pim-1 Oncogene (PIM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Pim-1 Oncogene (PIM1) level in the sample or standard.,
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pim-1 Oncogene (PIM1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pim-1 Oncogene (PIM1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all PIM1 CLIA Kits
    PIM1 (Pim-1 Oncogene (PIM1))
    Alternative Name
    Pim-1 Oncogene (PIM1 Products)
    Synonyms
    PIM CLIA Kit, Pim-1 CLIA Kit, PIM1 CLIA Kit, pim CLIA Kit, pim-1 CLIA Kit, pim3 CLIA Kit, Pim-1 proto-oncogene, serine/threonine kinase CLIA Kit, proviral integration site 1 CLIA Kit, Pim-1 proto-oncogene, serine/threonine kinase L homeolog CLIA Kit, PIM1 CLIA Kit, Pim1 CLIA Kit, pim1 CLIA Kit, pim1.L CLIA Kit
    Background

    Gene Name: Pim-1 Oncogene

    Gene Aliases: Proto-Oncogene Serine/Threonine-Protein Kinase Pim-1

    Gene ID
    5292
    UniProt
    P11309
    Pathways
    Glycosaminoglycan Metabolic Process
You are here:
Support