PSAT1 CLIA Kit

Catalog No. ABIN5657818

Product Details PSAT1 CLIA Kit

Target: PSAT1 (phosphoserine Aminotransferase 1 (PSAT1))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Phosphoserine Aminotransferase 1 (PSAT1). No significant cross-reactivity or interference between Phosphoserine Aminotransferase 1 (PSAT1) and analogues was observed.

Sensitivity: 0.059 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Phosphoserine Aminotransferase 1 (PSAT1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Phosphoserine Aminotransferase 1 (PSAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Phosphoserine Aminotransferase 1 (PSAT1) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphoserine Aminotransferase 1 (PSAT1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphoserine Aminotransferase 1 (PSAT1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: PSAT1 (phosphoserine Aminotransferase 1 (PSAT1))

Alternative Name: Phosphoserine Aminotransferase 1 (PSAT1 Products)

Synonyms: fi15b02 CLIA Kit, wu:fi15b02 CLIA Kit, zgc:55738 CLIA Kit, zgc:77622 CLIA Kit, EPIP CLIA Kit, PSA CLIA Kit, PSAT CLIA Kit, D8Ertd814e CLIA Kit, Psat CLIA Kit, Psa1 CLIA Kit, phosphoserine aminotransferase 1 CLIA Kit, phosphoserine aminotransferase 1 L homeolog CLIA Kit, psat1 CLIA Kit, psat1.L CLIA Kit, PSAT1 CLIA Kit, Psat1 CLIA Kit

Background:

Gene Name: Phosphoserine Aminotransferase 1

Gene Aliases: PSA, EPIP, Endometrial Progesterone Induced Protein, Phosphohydroxythreonine aminotransferase

Gene ID: 29968

UniProt: Q9Y617

Pathways: Warburg Effect