PIK3C3 ELISA Kit

Catalog No. ABIN5657783

Product Details PIK3C3 ELISA Kit

Target: PIK3C3 (Phosphoinositide-3-Kinase, Class 3 (PIK3C3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Phosphoinositide-3-Kinase Class 3 (PIK3C3). No significant cross-reactivity or interference between Phosphoinositide-3-Kinase Class 3 (PIK3C3) and analogues was observed.

Sensitivity: 0.59 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphoinositide-3-Kinase Class 3 (PIK3C3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphoinositide-3-Kinase Class 3 (PIK3C3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphoinositide-3-Kinase Class 3 (PIK3C3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphoinositide-3-Kinase Class 3 (PIK3C3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphoinositide-3-Kinase Class 3 (PIK3C3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphoinositide-3-Kinase Class 3 (PIK3C3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for PIK3C3

Target: PIK3C3 (Phosphoinositide-3-Kinase, Class 3 (PIK3C3))

Alternative Name: Phosphoinositide-3-Kinase Class 3 (PIK3C3 Products)

Synonyms: im:7147158 ELISA Kit, wu:fc13g06 ELISA Kit, wu:fz51e12 ELISA Kit, zgc:112016 ELISA Kit, zgc:113009 ELISA Kit, VPS34 ELISA Kit, hVps34 ELISA Kit, 5330434F23Rik ELISA Kit, Vps34 ELISA Kit, phosphatidylinositol 3-kinase catalytic subunit type 3 ELISA Kit, phosphatidylinositol 3-kinase catalytic subunit type 3 L homeolog ELISA Kit, phosphatidylinositol 3-kinase, catalytic subunit type 3 ELISA Kit, PIK3C3 ELISA Kit, pik3c3.L ELISA Kit, pik3c3 ELISA Kit, Pik3c3 ELISA Kit

Background:

Gene Name: Phosphoinositide-3-Kinase Class 3

Gene Aliases: Vps34, Phosphatidylinositol 3-kinase p100 subunit, Phosphatidylinositol 3-kinase catalytic subunit type 3

Gene ID: 5289

UniProt: Q8NEB9

Pathways: AMPK Signaling, Activation of Innate immune Response, Inositol Metabolic Process, Toll-Like Receptors Cascades, Autophagy