Peroxiredoxin 1 ELISA Kit

Catalog No. ABIN5657723

Product Details Peroxiredoxin 1 ELISA Kit

Target: Peroxiredoxin 1 (PRDX1)

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Peroxiredoxin 1 (PRDX1). No significant cross-reactivity or interference between Peroxiredoxin 1 (PRDX1) and analogues was observed.

Sensitivity: 0.063 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxiredoxin 1 (PRDX1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxiredoxin 1 (PRDX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxiredoxin 1 (PRDX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peroxiredoxin 1 (PRDX1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peroxiredoxin 1 (PRDX1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peroxiredoxin 1 (PRDX1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Peroxiredoxin 1

Target: Peroxiredoxin 1 (PRDX1)

Alternative Name: Peroxiredoxin 1 (PRDX1 Products)

Synonyms: MSP23 ELISA Kit, NKEF-A ELISA Kit, NKEFA ELISA Kit, PAG ELISA Kit, PAGA ELISA Kit, PAGB ELISA Kit, PRX1 ELISA Kit, PRXI ELISA Kit, TDPX2 ELISA Kit, NkefA ELISA Kit, OSF-3 ELISA Kit, OSF3 ELISA Kit, Paga ELISA Kit, PrdxI ELISA Kit, PrxI ELISA Kit, TDX2 ELISA Kit, TPxA ELISA Kit, Tdpx2 ELISA Kit, prx1 ELISA Kit, Hbp23 ELISA Kit, MGC80194 ELISA Kit, MGC84820 ELISA Kit, PRDX1 ELISA Kit, msp23 ELISA Kit, nkefa ELISA Kit, pag ELISA Kit, paga ELISA Kit, pagb ELISA Kit, prx-1 ELISA Kit, prxi ELISA Kit, tdpx2 ELISA Kit, hm:zehl0637 ELISA Kit, zgc:110343 ELISA Kit, TPX-2 ELISA Kit, peroxiredoxin 1 ELISA Kit, peroxiredoxin 1 S homeolog ELISA Kit, peroxiredoxin-1 pseudogene ELISA Kit, PRDX1 ELISA Kit, Prdx1 ELISA Kit, prdx1.S ELISA Kit, prdx1 ELISA Kit, LOC100350232 ELISA Kit

Background:

Gene Name: Peroxiredoxin 1

Gene Aliases: MSP23, NKEFA, PAG, PAGA, PAGB, TDPX2, Natural killer cell-enhancing factor A, Proliferation-associated, Thioredoxin peroxidase 2, Thioredoxin-dependent peroxide reductase 2

Pathways: p53 Signaling, EGFR Signaling Pathway, CXCR4-mediated Signaling Events