PIN1 ELISA Kit

Catalog No. ABIN5657687

Product Details PIN1 ELISA Kit

Target: PIN1 (Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 1 (PIN1). No significant cross-reactivity or interference between Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 1 (PIN1) and analogues was observed.

Sensitivity: 0.054 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 1 (PIN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 1 (PIN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 1 (PIN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 1 (PIN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 1 (PIN1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 1 (PIN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for PIN1

Target: PIN1 (Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1))

Alternative Name: Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 1 (PIN1 Products)

Synonyms: DOD ELISA Kit, UBL5 ELISA Kit, dod ELISA Kit, ubl5 ELISA Kit, pin1 ELISA Kit, pin1a ELISA Kit, 0610025L01Rik ELISA Kit, D9Bwg1161e ELISA Kit, pin1b ELISA Kit, zgc:73206 ELISA Kit, PPIase pin1 ELISA Kit, MdPin1 ELISA Kit, Pin1 ELISA Kit, peptidylprolyl cis/trans isomerase, NIMA-interacting 1 ELISA Kit, peptidylprolyl cis/trans isomerase, NIMA-interacting 1 S homeolog ELISA Kit, protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1 ELISA Kit, peptidylprolyl cis/trans isomerase, NIMA-interacting 1 L homeolog ELISA Kit, peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 ELISA Kit, peptidyl-prolyl cis-trans isomerase Pin1 ELISA Kit, PIN1 ELISA Kit, Pin1 ELISA Kit, pin1 ELISA Kit, pin1.S ELISA Kit, pin1.L ELISA Kit, ppiD ELISA Kit, PITG_11123 ELISA Kit, LOC103419188 ELISA Kit

Background:

Gene Name: Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 1

Gene Aliases: DOD, Protein(Peptidyl-Prolyl Cis/Trans Isomerase)NIMA-Interacting 1, Peptidyl-prolyl cis-trans isomerase Pin1, Rotamase Pin1

Gene ID: 5300

UniProt: Q13526