PAX6 ELISA Kit

Catalog No. ABIN5657602

Product Details PAX6 ELISA Kit

Target: PAX6 (Paired Box 6 (PAX6))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Paired Box Gene 6 (PAX6). No significant cross-reactivity or interference between Paired Box Gene 6 (PAX6) and analogues was observed.

Sensitivity: 0.057 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Paired Box Gene 6 (PAX6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Paired Box Gene 6 (PAX6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Paired Box Gene 6 (PAX6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Paired Box Gene 6 (PAX6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Paired Box Gene 6 (PAX6) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Paired Box Gene 6 (PAX6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for PAX6

Target: PAX6 (Paired Box 6 (PAX6))

Alternative Name: Paired Box Gene 6 (PAX6 Products)

Synonyms: pax6 ELISA Kit, an2 ELISA Kit, mgda ELISA Kit, wagr ELISA Kit, pax-6 ELISA Kit, pax6a ELISA Kit, xpax6 ELISA Kit, XLPAX6 ELISA Kit, MGC52531 ELISA Kit, PAX-6 ELISA Kit, AN ELISA Kit, AN2 ELISA Kit, D11S812E ELISA Kit, MGDA ELISA Kit, WAGR ELISA Kit, Pax-6 ELISA Kit, pax6b ELISA Kit, 1500038E17Rik ELISA Kit, AEY11 ELISA Kit, Dey ELISA Kit, Gsfaey11 ELISA Kit, Sey ELISA Kit, PAXZF-A ELISA Kit, Pax6.1 ELISA Kit, cb280 ELISA Kit, fc20e07 ELISA Kit, pax-a ELISA Kit, pax[zf-a] ELISA Kit, paxzfa ELISA Kit, wu:fc20e07 ELISA Kit, zfpax-6a ELISA Kit, zfpax-6b ELISA Kit, paired box 6 ELISA Kit, paired box 6 L homeolog ELISA Kit, paired box 6 S homeolog ELISA Kit, paired box 6a ELISA Kit, PAX6 ELISA Kit, pax6 ELISA Kit, Pax6 ELISA Kit, pax6.L ELISA Kit, pax6.S ELISA Kit, pax6a ELISA Kit

Background:

Gene Name: Paired Box Gene 6

Gene Aliases: AN, AN2, MGDA, WAGR, Oculorhombin, Aniridia,Keratitis, Aniridia type II protein

Gene ID: 25509

UniProt: P63016

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis