OGG1 ELISA Kit

Catalog No. ABIN5657584

Product Details OGG1 ELISA Kit

Target: OGG1 (8-Oxoguanine DNA Glycosylase (OGG1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.25 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Oxoguanine Glycosylase 1 (OGG1). No significant cross-reactivity or interference between Oxoguanine Glycosylase 1 (OGG1) and analogues was observed.

Sensitivity: 12.7 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Oxoguanine Glycosylase 1 (OGG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Oxoguanine Glycosylase 1 (OGG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Oxoguanine Glycosylase 1 (OGG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Oxoguanine Glycosylase 1 (OGG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Oxoguanine Glycosylase 1 (OGG1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Oxoguanine Glycosylase 1 (OGG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for OGG1

Target: OGG1 (8-Oxoguanine DNA Glycosylase (OGG1))

Alternative Name: Oxoguanine Glycosylase 1 (OGG1 Products)

Synonyms: OGG1 ELISA Kit, zgc:158858 ELISA Kit, 8-oxoguanine-DNA glycosylase 1 ELISA Kit, ARABIDOPSIS 8-OXOGUANINE-DNA GLYCOSYLASE 1 ELISA Kit, ATOGG1 ELISA Kit, F8K7.14 ELISA Kit, F8K7_14 ELISA Kit, 19.m02329 ELISA Kit, DDBDRAFT_0184479 ELISA Kit, DDBDRAFT_0233024 ELISA Kit, DDB_0184479 ELISA Kit, DDB_0233024 ELISA Kit, ogg1 ELISA Kit, Tb04.1H19.930 ELISA Kit, HMMH ELISA Kit, HOGG1 ELISA Kit, MUTM ELISA Kit, OGH1 ELISA Kit, Mmh ELISA Kit, 8-oxoguanine DNA glycosylase ELISA Kit, 8-oxoguanine DNA glycosylase L homeolog ELISA Kit, 8-oxoguanine-DNA glycosylase 1 ELISA Kit, 8-OXOGUANINE DNA GLYCOSYLASE ELISA Kit, putative 8-oxoguanine DNA glycosylase ELISA Kit, 8-oxoguanine DNA-glycosylase ELISA Kit, N-glycosylase/DNA lyase OGG1 ELISA Kit, 8-oxoguanine DNA-glycosylase 1 ELISA Kit, OGG1 ELISA Kit, ogg1.L ELISA Kit, ogg1 ELISA Kit, ECU08_0770 ELISA Kit, CA_C2707 ELISA Kit, BBOV_I004290 ELISA Kit, Smp_074010.1 ELISA Kit, LOC9329243 ELISA Kit, Tb927.4.2480 ELISA Kit, Ogg1 ELISA Kit

Background:

Gene Name: Oxoguanine Glycosylase 1

Gene Aliases: HMMH, HOGG1, MUTM, OGH1, 8-Oxoguanine DNA Glycosylase, DNA-(apurinic or apyrimidinic site) lyase, N-glycosylase/DNA lyase

Gene ID: 18294

UniProt: O08760

Pathways: DNA Damage Repair