Ovalbumin ELISA Kit

Catalog No. ABIN5657568

Product Details Ovalbumin ELISA Kit

Target: Ovalbumin (OVA)

Reactivity: Various Species

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78 pg/mL - 5000 pg/mL

Minimum Detection Limit: 78 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Ovalbumin (OVA). No significant cross-reactivity or interference between Ovalbumin (OVA) and analogues was observed.

Sensitivity: 30 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ovalbumin (OVA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ovalbumin (OVA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ovalbumin (OVA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ovalbumin (OVA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ovalbumin (OVA) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ovalbumin (OVA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Ovalbumin

Target: Ovalbumin (OVA)

Alternative Name: Ovalbumin (OVA Products)

Synonyms: 1600019A21Rik ELISA Kit, R86 ELISA Kit, SPI-CI ELISA Kit, Spi10 ELISA Kit, ovalbumin ELISA Kit, NK13 ELISA Kit, Spi12 ELISA Kit, BC052216 ELISA Kit, NK21 ELISA Kit, NK21L1 ELISA Kit, Spi13 ELISA Kit, NK26 ELISA Kit, Spi14 ELISA Kit, AW540195 ELISA Kit, AT2 ELISA Kit, Spi9 ELISA Kit, EID ELISA Kit, SPI3B ELISA Kit, Gm11396 ELISA Kit, OTTMUSG00000000720 ELISA Kit, SERPINB14 ELISA Kit, C76171 ELISA Kit, C76174 ELISA Kit, SPI3C ELISA Kit, SPIC ELISA Kit, serine (or cysteine) peptidase inhibitor, clade B, member 9b ELISA Kit, serine (or cysteine) peptidase inhibitor, clade B, member 6b ELISA Kit, serine (or cysteine) peptidase inhibitor, clade B, member 9f ELISA Kit, serine (or cysteine) peptidase inhibitor, clade B, member 9e ELISA Kit, serine (or cysteine) peptidase inhibitor, clade B, member 9d ELISA Kit, serine (or cysteine) peptidase inhibitor, clade B, member 1, pseudogene ELISA Kit, serine (or cysteine) peptidase inhibitor, clade B, member 6e ELISA Kit, ovalbumin (SERPINB14) ELISA Kit, serine (or cysteine) peptidase inhibitor, clade B, member 6c ELISA Kit, Serpinb9b ELISA Kit, Serpinb6b ELISA Kit, Serpinb9f ELISA Kit, Serpinb9e ELISA Kit, Serpinb9d ELISA Kit, Serpinb1-ps1 ELISA Kit, Serpinb6e ELISA Kit, OVAL ELISA Kit, Serpinb6c ELISA Kit

Target Type: Virus

Background:

Gene Name: Ovalbumin