OAT ELISA Kit

Catalog No. ABIN5657518

Product Details OAT ELISA Kit

Target: OAT (Ornithine Aminotransferase (OAT))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.625 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.625 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Ornithine Aminotransferase (OAT). No significant cross-reactivity or interference between Ornithine Aminotransferase (OAT) and analogues was observed.

Sensitivity: 0.253 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ornithine Aminotransferase (OAT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ornithine Aminotransferase (OAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ornithine Aminotransferase (OAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ornithine Aminotransferase (OAT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ornithine Aminotransferase (OAT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ornithine Aminotransferase (OAT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for OAT

Target: OAT (Ornithine Aminotransferase (OAT))

Alternative Name: Ornithine Aminotransferase (OAT Products)

Synonyms: 151832_at ELISA Kit, CG8782 ELISA Kit, Dmel\\CG8782 ELISA Kit, hoga ELISA Kit, AI194874 ELISA Kit, rOAT ELISA Kit, GACR ELISA Kit, HOGA ELISA Kit, OATASE ELISA Kit, OKT ELISA Kit, Ornithine aminotransferase precursor ELISA Kit, ornithine aminotransferase, gene 1 ELISA Kit, ornithine aminotransferase ELISA Kit, Oat ELISA Kit, oat.1 ELISA Kit, OAT ELISA Kit, Lbys_2951 ELISA Kit, Riean_0998 ELISA Kit, Ftrac_2019 ELISA Kit, Celal_2605 ELISA Kit, Deipr_1149 ELISA Kit, Celly_0230 ELISA Kit, Weevi_2065 ELISA Kit, Fluta_4071 ELISA Kit, Halhy_2848 ELISA Kit, Theth_0565 ELISA Kit, oat ELISA Kit

Background:

Gene Name: Ornithine Aminotransferase

Gene Aliases: HOGA, Grate Arophy, Ornithine delta-aminotransferase, Ornithine--oxo-acid aminotransferase

Gene ID: 4942

UniProt: P04181