Orexin ELISA Kit

Catalog No. ABIN5657512

Product Details Orexin ELISA Kit

Target: Orexin (OX)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 6.17 pg/mL - 500 pg/mL

Minimum Detection Limit: 6.17 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Orexin (OX). No significant cross-reactivity or interference between Orexin (OX) and analogues was observed.

Sensitivity: 2.65 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 h

Plate: Pre-coated

Protocol: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Orexin (OX) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Orexin (OX) and unlabeled Orexin (OX) (Standards or samples) with the pre-coated antibody specific to Orexin (OX). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Orexin (OX) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Orexin (OX) in the sample.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Orexin (OX) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Orexin (OX) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Orexin

Target: Orexin (OX)

Alternative Name: Orexin (OX Products)

Synonyms: orexin-A ELISA Kit, PPOX ELISA Kit, NRCLP1 ELISA Kit, OX ELISA Kit, hypocretin neuropeptide precursor ELISA Kit, hypocretin ELISA Kit, Hcrt ELISA Kit, HCRT ELISA Kit

Background:

Gene Name: Orexin

Gene Aliases: HCRT, PPOX, Orexin A, Hypocretin 1, Orexin B, Hypocretin 2, Preproorexin, Hypocretin Neuropeptide Precursor

Pathways: Synaptic Membrane, Feeding Behaviour