OLIG2 ELISA Kit

Catalog No. ABIN5657498

Product Details OLIG2 ELISA Kit

Target: OLIG2 (Oligodendrocyte Lineage Transcription Factor 2 (OLIG2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.62 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.62 pg/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Oligodendrocyte Lineage Transcription Factor 2 (OLIG2). No significant cross-reactivity or interference between Oligodendrocyte Lineage Transcription Factor 2 (OLIG2) and analogues was observed.

Sensitivity: 5.7 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Oligodendrocyte Lineage Transcription Factor 2 (OLIG2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Oligodendrocyte Lineage Transcription Factor 2 (OLIG2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Oligodendrocyte Lineage Transcription Factor 2 (OLIG2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Oligodendrocyte Lineage Transcription Factor 2 (OLIG2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Oligodendrocyte Lineage Transcription Factor 2 (OLIG2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Oligodendrocyte Lineage Transcription Factor 2 (OLIG2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for OLIG2

Target: OLIG2 (Oligodendrocyte Lineage Transcription Factor 2 (OLIG2))

Alternative Name: Oligodendrocyte Lineage Transcription Factor 2 (OLIG2 Products)

Synonyms: olig3.2 ELISA Kit, MGC89336 ELISA Kit, OLIG2 ELISA Kit, BHLHB1 ELISA Kit, OLIGO2 ELISA Kit, PRKCBP2 ELISA Kit, RACK17 ELISA Kit, bHLHe19 ELISA Kit, wu:fc64g09 ELISA Kit, AI604895 ELISA Kit, Bhlhb1 ELISA Kit, Olg-2 ELISA Kit, Oligo2 ELISA Kit, RK17 ELISA Kit, oligodendrocyte transcription factor 4 ELISA Kit, oligodendrocyte transcription factor 2 ELISA Kit, oligodendrocyte lineage transcription factor 2 ELISA Kit, olig4 ELISA Kit, OLIG2 ELISA Kit, Olig2 ELISA Kit, olig2 ELISA Kit

Background:

Gene Name: Oligodendrocyte Lineage Transcription Factor 2

Gene Aliases: BHLHB1, OLIGO2, PRKCBP2, RACK17, Basic Domain,Helix-Loop-Helix Protein,Class B 1, Class B basic helix-loop-helix protein 1, Class E basic helix-loop-helix protein 19