OGT ELISA Kit

Catalog No. ABIN5657483

Product Details OGT ELISA Kit

Target: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of O-Linked-N-Acetylglucosamine Transferase (OGT). No significant cross-reactivity or interference between O-Linked-N-Acetylglucosamine Transferase (OGT) and analogues was observed.

Sensitivity: 0.59 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to O-Linked-N-Acetylglucosamine Transferase (OGT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to O-Linked-N-Acetylglucosamine Transferase (OGT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain O-Linked-N-Acetylglucosamine Transferase (OGT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of O-Linked-N-Acetylglucosamine Transferase (OGT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level O-Linked-N-Acetylglucosamine Transferase (OGT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level O-Linked-N-Acetylglucosamine Transferase (OGT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for OGT

Target: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Alternative Name: O-Linked-N-Acetylglucosamine Transferase (OGT Products)

Synonyms: BcDNA:GH04245 ELISA Kit, CG10392 ELISA Kit, Dmel\\CG10392 ELISA Kit, OGT ELISA Kit, Ogt ELISA Kit, P1201 ELISA Kit, SXC ELISA Kit, Sxc ELISA Kit, l(2)02637 ELISA Kit, l(2)NC130 ELISA Kit, sxc/Ogt ELISA Kit, fm81g08 ELISA Kit, ogt ELISA Kit, wu:fc12b01 ELISA Kit, wu:fm81g08 ELISA Kit, HRNT1 ELISA Kit, O-GLCNAC ELISA Kit, 1110038P24Rik ELISA Kit, 4831420N21Rik ELISA Kit, AI115525 ELISA Kit, Ogtl ELISA Kit, O-linked N-acetylglucosamine (GlcNAc) transferase L homeolog ELISA Kit, super sex combs ELISA Kit, O-linked N-acetylglucosamine (GlcNAc) transferase, tandem duplicate 1 ELISA Kit, O-linked N-acetylglucosamine (GlcNAc) transferase ELISA Kit, O-linked GlcNAc transferase ELISA Kit, o-linked GlcNAc transferase ELISA Kit, O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) ELISA Kit, ogt.L ELISA Kit, sxc ELISA Kit, ogt.1 ELISA Kit, OGT ELISA Kit, ogt ELISA Kit, GL50803_12081 ELISA Kit, GL50803_41701 ELISA Kit, THAPSDRAFT_264441 ELISA Kit, spyA ELISA Kit, Ogt ELISA Kit

Background:

Gene Name: O-Linked-N-Acetylglucosamine Transferase

Gene Aliases: HRNT1, O-GLCNAC, UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit, O-GlcNAc transferase subunit p110

Pathways: Regulation of Carbohydrate Metabolic Process