NAP1L1 ELISA Kit

Catalog No. ABIN5657477

Product Details NAP1L1 ELISA Kit

Target: NAP1L1 (Nucleosome Assembly Protein 1-Like 1 (NAP1L1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Nucleosome Assembly Protein 1 Like Protein 1 (NAP1L1). No significant cross-reactivity or interference between Nucleosome Assembly Protein 1 Like Protein 1 (NAP1L1) and analogues was observed.

Sensitivity: 0.119 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nucleosome Assembly Protein 1 Like Protein 1 (NAP1L1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nucleosome Assembly Protein 1 Like Protein 1 (NAP1L1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nucleosome Assembly Protein 1 Like Protein 1 (NAP1L1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nucleosome Assembly Protein 1 Like Protein 1 (NAP1L1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nucleosome Assembly Protein 1 Like Protein 1 (NAP1L1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nucleosome Assembly Protein 1 Like Protein 1 (NAP1L1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for NAP1L1

Target: NAP1L1 (Nucleosome Assembly Protein 1-Like 1 (NAP1L1))

Alternative Name: Nucleosome Assembly Protein 1 Like Protein 1 (NAP1L1 Products)

Synonyms: NAP1 ELISA Kit, NAP1L ELISA Kit, NRP ELISA Kit, cb716 ELISA Kit, sb:cb13 ELISA Kit, wu:fb83g07 ELISA Kit, nap1-B ELISA Kit, nap1l1 ELISA Kit, xNAP-1 ELISA Kit, xNAP1L-B ELISA Kit, xnap1l ELISA Kit, xnap1l1 ELISA Kit, nap1 ELISA Kit, nap1l ELISA Kit, nrp ELISA Kit, AA407126 ELISA Kit, AI256722 ELISA Kit, D10Ertd68e ELISA Kit, NAP-1 ELISA Kit, ARABIDOPSIS THALIANA NUCLEOSOME ASSEMLY PROTEIN 1;1 ELISA Kit, ATNAP1;1 ELISA Kit, F20B18.220 ELISA Kit, F20B18_220 ELISA Kit, nucleosome assembly protein1;1 ELISA Kit, nap1-A ELISA Kit, xNAP1 ELISA Kit, xNAP1L-A ELISA Kit, nucleosome assembly protein 1 like 1 ELISA Kit, nucleosome assembly protein 1-like 1 ELISA Kit, nucleosome assembly protein 1 like 1 L homeolog ELISA Kit, nucleosome assembly protein1;1 ELISA Kit, nucleosome assembly protein 1 like 1 S homeolog ELISA Kit, NAP1L1 ELISA Kit, nap1l1 ELISA Kit, nap1l1.L ELISA Kit, Nap1l1 ELISA Kit, NAP1;1 ELISA Kit, nap1l1.S ELISA Kit

Background:

Gene Name: Nucleosome Assembly Protein 1 Like Protein 1

Gene Aliases: NAP1, NAP1L, NRP, NAP-1-related protein

Gene ID: 4673

UniProt: P55209