NUP62 ELISA Kit

Catalog No. ABIN5657474

Product Details NUP62 ELISA Kit

Target: NUP62 (Nucleoporin 62kDa (NUP62))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Nucleoporin 62 kDa (NUP62). No significant cross-reactivity or interference between Nucleoporin 62 kDa (NUP62) and analogues was observed.

Sensitivity: 0.112 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nucleoporin 62 kDa (NUP62). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nucleoporin 62 kDa (NUP62). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nucleoporin 62 kDa (NUP62), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nucleoporin 62 kDa (NUP62) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nucleoporin 62kDa (NUP62) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nucleoporin 62kDa (NUP62) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for NUP62

Target: NUP62 (Nucleoporin 62kDa (NUP62))

Alternative Name: Nucleoporin 62kDa (NUP62 Products)

Synonyms: IBSN ELISA Kit, SNDI ELISA Kit, p62 ELISA Kit, NUP62 ELISA Kit, AA589433 ELISA Kit, AI426861 ELISA Kit, AI790512 ELISA Kit, AU045898 ELISA Kit, D7Ertd649e ELISA Kit, Nupc1 ELISA Kit, CG6251 ELISA Kit, Dmel\\CG6251 ELISA Kit, DDBDRAFT_0167902 ELISA Kit, DDBDRAFT_0237712 ELISA Kit, DDB_0167902 ELISA Kit, DDB_0237712 ELISA Kit, nup62 ELISA Kit, Np62 ELISA Kit, nucleoporin 62 ELISA Kit, hypothetical protein ELISA Kit, nucleoporin 62kDa ELISA Kit, Nucleoporin 62kD ELISA Kit, predicted protein ELISA Kit, nuclear pore protein ELISA Kit, Nup62 nuclear pore complex glycoprotein p62-related protein (IC) ELISA Kit, Nuclear pore glycoprotein p62 ELISA Kit, NUP62 ELISA Kit, PGTG_03077 ELISA Kit, Nup62 ELISA Kit, nup62 ELISA Kit

Background:

Gene Name: Nucleoporin 62 kDa

Gene Aliases: IBSN, p62, SNDI, Nuclear Pore Glycoprotein p62

Gene ID: 23636

UniProt: P37198

Pathways: EGFR Signaling Pathway, SARS-CoV-2 Protein Interactome