NCOA3 ELISA Kit

Catalog No. ABIN5657459

Product Details NCOA3 ELISA Kit

Target: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Nuclear Receptor Coactivator 3 (NCOA3). No significant cross-reactivity or interference between Nuclear Receptor Coactivator 3 (NCOA3) and analogues was observed.

Sensitivity: 0.124 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Receptor Coactivator 3 (NCOA3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Receptor Coactivator 3 (NCOA3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Receptor Coactivator 3 (NCOA3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nuclear Receptor Coactivator 3 (NCOA3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nuclear Receptor Coactivator 3 (NCOA3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nuclear Receptor Coactivator 3 (NCOA3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for NCOA3

Target: NCOA3 (Nuclear Receptor Coactivator 3 (NCOA3))

Alternative Name: Nuclear Receptor Coactivator 3 (NCOA3 Products)

Synonyms: NCOA3 ELISA Kit, ACTR ELISA Kit, AIB-1 ELISA Kit, AIB1 ELISA Kit, CAGH16 ELISA Kit, CTG26 ELISA Kit, KAT13B ELISA Kit, RAC3 ELISA Kit, SRC-3 ELISA Kit, SRC3 ELISA Kit, TNRC14 ELISA Kit, TNRC16 ELISA Kit, TRAM-1 ELISA Kit, bHLHe42 ELISA Kit, pCIP ELISA Kit, 2010305B15Rik ELISA Kit, AW321064 ELISA Kit, Actr ELISA Kit, Aib1 ELISA Kit, Rac3 ELISA Kit, Src3 ELISA Kit, Tram-1 ELISA Kit, Tram1 ELISA Kit, p/Cip ELISA Kit, pCip ELISA Kit, actr ELISA Kit, aib-1 ELISA Kit, aib1 ELISA Kit, cagh16 ELISA Kit, ctg26 ELISA Kit, kat13b ELISA Kit, pcip ELISA Kit, rac3 ELISA Kit, src-3 ELISA Kit, src3 ELISA Kit, tnrc14 ELISA Kit, tnrc16 ELISA Kit, tram-1 ELISA Kit, xSRC-3 ELISA Kit, nuclear receptor coactivator 3 ELISA Kit, nuclear receptor coactivator 3 S homeolog ELISA Kit, NCOA3 ELISA Kit, Ncoa3 ELISA Kit, ncoa3.S ELISA Kit

Background:

Gene Name: Nuclear Receptor Coactivator 3

Gene Aliases: RAC3, ACTR, AIB1, CAGH16, CTG26, SRC3, TNRC14, TNRC16, TRAM1, pCIP, Amplified In Breast 1, Steroid Receptor Coactivator-3 , Thyroid Hormone Receptor Activator Molecule 1

Gene ID: 8202

UniProt: Q9Y6Q9

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha