Nischarin ELISA Kit

Catalog No. ABIN5657396

Product Details Nischarin ELISA Kit

Target: Nischarin (NISCH)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Nischarin (NISCH). No significant cross-reactivity or interference between Nischarin (NISCH) and analogues was observed.

Sensitivity: 0.054 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nischarin (NISCH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nischarin (NISCH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nischarin (NISCH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nischarin (NISCH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nischarin (NISCH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nischarin (NISCH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Nischarin

Target: Nischarin (NISCH)

Alternative Name: Nischarin (NISCH Products)

Synonyms: NISCH ELISA Kit, IRAS ELISA Kit, 1200007D05Rik ELISA Kit, 3202002H23Rik ELISA Kit, AW494485 ELISA Kit, I-1 ELISA Kit, IR1 ELISA Kit, hIRAS ELISA Kit, nischarin ELISA Kit, nischarin S homeolog ELISA Kit, NISCH ELISA Kit, Cyan7822_6841 ELISA Kit, nisch.S ELISA Kit, nisch ELISA Kit, Nisch ELISA Kit

Background:

Gene Name: Nischarin

Gene Aliases: IRAS, Imidazoline Receptor Candidate, I-1 Receptor Candidate Protein, Imidazoline Receptor Antisera Selected, Imidazoline receptor antisera-selected protein

Gene ID: 11188

UniProt: Q9Y2I1