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Nidogen ELISA Kit

NID Reactivity: Human Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum
Catalog No. ABIN5657390
  • Target See all Nidogen (NID) products
    Nidogen (NID)
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.781 ng/mL - 50 ng/mL
    Minimum Detection Limit
    0.781 ng/mL
    Application
    ELISA
    Sample Type
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Nidogen (NID). No significant cross-reactivity or interference between Nidogen (NID) and analogues was observed.
    Sensitivity
    0.33 ng/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nidogen (NID). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nidogen (NID). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nidogen (NID), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nidogen (NID) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nidogen (NID) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nidogen (NID) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all Nidogen (NID) products
    Nidogen (NID)
    Alternative Name
    Nidogen (NID Products)
    Synonyms
    BEST:CK00552 ELISA Kit, CG12908 ELISA Kit, CT32053 ELISA Kit, Dmel\\CG12908 ELISA Kit, ndg ELISA Kit, Nidogen/entactin ELISA Kit, Ndg ELISA Kit
    Background

    Gene Name: Nidogen

    Gene Aliases: Entactin

    Gene ID
    4811
    UniProt
    P14543
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