NEUROD6 ELISA Kit

Catalog No. ABIN5657278

Product Details NEUROD6 ELISA Kit

Target: NEUROD6 (Neurogenic Differentiation 6 (NEUROD6))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Neurogenic Differentiation 6 (NEUROD6). No significant cross-reactivity or interference between Neurogenic Differentiation 6 (NEUROD6) and analogues was observed.

Sensitivity: 0.055 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neurogenic Differentiation 6 (NEUROD6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neurogenic Differentiation 6 (NEUROD6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neurogenic Differentiation 6 (NEUROD6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neurogenic Differentiation 6 (NEUROD6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neurogenic Differentiation 6 (NEUROD6) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neurogenic Differentiation 6 (NEUROD6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for NEUROD6

Target: NEUROD6 (Neurogenic Differentiation 6 (NEUROD6))

Alternative Name: Neurogenic Differentiation 6 (NEUROD6 Products)

Synonyms: Atoh2 ELISA Kit, MATH2 ELISA Kit, Math-2 ELISA Kit, NEX1M ELISA Kit, Nex1 ELISA Kit, bHLHa2 ELISA Kit, nex1 ELISA Kit, Xath2 ELISA Kit, atoh2 ELISA Kit, math2 ELISA Kit, nex1m ELISA Kit, bhlha2 ELISA Kit, math-2 ELISA Kit, neurod6-A ELISA Kit, RGD1562793 ELISA Kit, MGC145341 ELISA Kit, Math2 ELISA Kit, Nex ELISA Kit, Nex1m ELISA Kit, atoh2b ELISA Kit, ndr1b ELISA Kit, zNdr1b ELISA Kit, zgc:92543 ELISA Kit, NeuroD6 ELISA Kit, NeuroD6-A ELISA Kit, Zath2a ELISA Kit, atoh2a ELISA Kit, ndr1a ELISA Kit, neuroD6-A ELISA Kit, neuronal differentiation 6 ELISA Kit, neuronal differentiation 6 L homeolog ELISA Kit, neurogenic differentiation 6 ELISA Kit, neuronal differentiation 6b ELISA Kit, neuronal differentiation 6a ELISA Kit, NEUROD6 ELISA Kit, neurod6.L ELISA Kit, Neurod6 ELISA Kit, neurod6 ELISA Kit, neurod6b ELISA Kit, neurod6a ELISA Kit

Background:

Gene Name: Neurogenic Differentiation 6

Gene Aliases: Atoh2, NEX1M, Math-2, bHLHa2, Neurogenic differentiation factor 6, Mammalian Atonal Homolog 2, Atonal Homolog 2, Class A basic helix-loop-helix protein 2