NEFM ELISA Kit

Catalog No. ABIN5657265

Product Details NEFM ELISA Kit

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.62 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.62 pg/mL

Application: ELISA

Sample Type: Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Neurofilament 3 (NEF3). No significant cross-reactivity or interference between Neurofilament 3 (NEF3) and analogues was observed.

Sensitivity: 6.2 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neurofilament 3 (NEF3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neurofilament 3 (NEF3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neurofilament 3 (NEF3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neurofilament 3 (NEF3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neurofilament 3 (NEF3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neurofilament 3 (NEF3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for NEFM

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Alternative Name: Neurofilament 3 (NEFM Products)

Synonyms: NEF3 ELISA Kit, NF-M ELISA Kit, NFM ELISA Kit, Nef3 ELISA Kit, Nfm ELISA Kit, NF160 ELISA Kit, NF165 ELISA Kit, nef-m ELISA Kit, nef3 ELISA Kit, nefm ELISA Kit, nf-m ELISA Kit, zgc:112359 ELISA Kit, nefm-a ELISA Kit, nefm-b ELISA Kit, neurofilament medium ELISA Kit, neurofilament triplet M protein ELISA Kit, neurofilament, medium polypeptide ELISA Kit, neurofilament medium polypeptide ELISA Kit, neurofilament medium L homeolog ELISA Kit, neurofilament, medium polypeptide a ELISA Kit, neurofilament medium S homeolog ELISA Kit, NEFM ELISA Kit, Nefm ELISA Kit, TVAG_582010 ELISA Kit, TVAG_379630 ELISA Kit, nefm.L ELISA Kit, nefma ELISA Kit, nefm.S ELISA Kit

Background:

Gene Name: Neurofilament 3

Gene Aliases: NEFM, NF-M, NFM, Neurofilament,Medium Polypeptide, 160 kDa neurofilament protein, Neurofilament triplet M protein

Gene ID: 4741

UniProt: P07197

Pathways: Brown Fat Cell Differentiation