NEIL3 ELISA Kit

Catalog No. ABIN5657201

Product Details NEIL3 ELISA Kit

Target: NEIL3 (Nei Endonuclease VIII-Like 3 (NEIL3))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Nei Endonuclease VIII Like Protein 3 (NEIL3). No significant cross-reactivity or interference between Nei Endonuclease VIII Like Protein 3 (NEIL3) and analogues was observed.

Sensitivity: 0.061 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nei Endonuclease VIII Like Protein 3 (NEIL3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nei Endonuclease VIII Like Protein 3 (NEIL3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nei Endonuclease VIII Like Protein 3 (NEIL3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nei Endonuclease VIII Like Protein 3 (NEIL3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nei Endonuclease VIII Like Protein 3 (NEIL3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nei Endonuclease VIII Like Protein 3 (NEIL3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for NEIL3

Target: NEIL3 (Nei Endonuclease VIII-Like 3 (NEIL3))

Alternative Name: Nei Endonuclease VIII Like Protein 3 (NEIL3 Products)

Synonyms: RGD1310562 ELISA Kit, NEIL3 ELISA Kit, zgc:103553 ELISA Kit, neil3 ELISA Kit, MAC12.12 ELISA Kit, MAC12_12 ELISA Kit, FGP2 ELISA Kit, FPG2 ELISA Kit, NEI3 ELISA Kit, hFPG2 ELISA Kit, hNEI3 ELISA Kit, AI449477 ELISA Kit, BC034753 ELISA Kit, C85903 ELISA Kit, nei-like DNA glycosylase 3 ELISA Kit, nei like DNA glycosylase 3 ELISA Kit, GRF zinc finger / Zinc knuckle protein ELISA Kit, nei-like DNA glycosylase 3 L homeolog ELISA Kit, nei like 3 (E. coli) ELISA Kit, Neil3 ELISA Kit, NEIL3 ELISA Kit, neil3 ELISA Kit, AT5G13920 ELISA Kit, neil3.L ELISA Kit

Background:

Gene Name: Nei Endonuclease VIII Like Protein 3

Gene Aliases: FPG2, hFPG2, hNEI3, Endonuclease 8-like 3, DNA glycosylase FPG2, DNA glycosylase/AP lyase Neil3, Nei-like protein 3

Gene ID: 234258

UniProt: Q8K203

Pathways: DNA Damage Repair