NPR1 ELISA Kit

Catalog No. ABIN5657182

Product Details NPR1 ELISA Kit

Target: NPR1 (Natriuretic Peptide Receptor A/guanylate Cyclase A (Atrionatriuretic Peptide Receptor A) (NPR1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Natriuretic Peptide Receptor 1 (NPR1). No significant cross-reactivity or interference between Natriuretic Peptide Receptor 1 (NPR1) and analogues was observed.

Sensitivity: 0.115 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Natriuretic Peptide Receptor 1 (NPR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Natriuretic Peptide Receptor 1 (NPR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Natriuretic Peptide Receptor 1 (NPR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Natriuretic Peptide Receptor 1 (NPR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Natriuretic Peptide Receptor 1 (NPR1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Natriuretic Peptide Receptor 1 (NPR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for NPR1

Target: NPR1 (Natriuretic Peptide Receptor A/guanylate Cyclase A (Atrionatriuretic Peptide Receptor A) (NPR1))

Alternative Name: Natriuretic Peptide Receptor 1 (NPR1 Products)

Synonyms: ANPRA ELISA Kit, ANPa ELISA Kit, GUC2A ELISA Kit, GUCY2A ELISA Kit, NPRA ELISA Kit, Anpra ELISA Kit, Gca ELISA Kit, NPR-A ELISA Kit, Npra ELISA Kit, sGC ELISA Kit, si:dkey-27c15.1 ELISA Kit, AI893888 ELISA Kit, GC-A ELISA Kit, Pndr ELISA Kit, ARABIDOPSIS NONEXPRESSER OF PR GENES 1 ELISA Kit, ATNPR1 ELISA Kit, F15H21.6 ELISA Kit, F15H21_6 ELISA Kit, NIM1 ELISA Kit, NON-INDUCIBLE IMMUNITY 1 ELISA Kit, NONEXPRESSER OF PR GENES 1 ELISA Kit, REGULATORY PROTEIN NPR1 ELISA Kit, SAI1 ELISA Kit, SALICYLIC ACID INSENSITIVE 1 ELISA Kit, natriuretic peptide receptor 1 ELISA Kit, natriuretic peptide receptor 1a ELISA Kit, regulatory protein (NPR1) ELISA Kit, NPR1 ELISA Kit, Npr1 ELISA Kit, npr1a ELISA Kit

Background:

Gene Name: Natriuretic Peptide Receptor 1

Gene Aliases: ANPRA, ANPa, GUC2A, GUCY2A, NPR-A, Guanylate Cyclase A, Atrionatriuretic Peptide Receptor A, Atrial natriuretic peptide receptor type A