MARCKS ELISA Kit

Catalog No. ABIN5657127

Product Details MARCKS ELISA Kit

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS). No significant cross-reactivity or interference between Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS) and analogues was observed.

Sensitivity: 0.55 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for MARCKS

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Alternative Name: Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS Products)

Synonyms: 80K-L ELISA Kit, MACS ELISA Kit, PKCSL ELISA Kit, PRKCSL ELISA Kit, KINC ELISA Kit, Macs ELISA Kit, marcks ELISA Kit, MGC89127 ELISA Kit, MARCKS ELISA Kit, macs ELISA Kit, wu:fb55c06 ELISA Kit, wu:fb92d04 ELISA Kit, wu:fc19b04 ELISA Kit, wu:fc44a10 ELISA Kit, zgc:110235 ELISA Kit, fc34g03 ELISA Kit, fc38c06 ELISA Kit, wu:fc34g03 ELISA Kit, wu:fc38c06 ELISA Kit, zgc:109978 ELISA Kit, myristoylated alanine rich protein kinase C substrate ELISA Kit, myristoylated alanine-rich protein kinase C substrate ELISA Kit, methyl binding domain106 ELISA Kit, myristoylated alanine-rich protein kinase C substrate b ELISA Kit, myristoylated alanine-rich protein kinase C substrate S homeolog ELISA Kit, myristoylated alanine-rich protein kinase C substrate a ELISA Kit, MARCKS ELISA Kit, Marcks ELISA Kit, marcks ELISA Kit, mbd106 ELISA Kit, marcksb ELISA Kit, marcks.S ELISA Kit, marcksa ELISA Kit

Background:

Gene Name: Myristoylated Alanine Rich Protein Kinase C Substrate

Gene Aliases: 80K-L, MACS, MRACKS, PKCSL, PRKCSL, Protein kinase C substrate, 80 kDa protein, light chain