MYLK ELISA Kit

Catalog No. ABIN5657108

Product Details MYLK ELISA Kit

Target: MYLK (Myosin Light Chain Kinase (MYLK))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Myosin Light Chain Kinase (MYLK). No significant cross-reactivity or interference between Myosin Light Chain Kinase (MYLK) and analogues was observed.

Sensitivity: 0.059 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Light Chain Kinase (MYLK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Light Chain Kinase (MYLK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Light Chain Kinase (MYLK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myosin Light Chain Kinase (MYLK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myosin Light Chain Kinase (MYLK) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myosin Light Chain Kinase (MYLK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for MYLK

Target: MYLK (Myosin Light Chain Kinase (MYLK))

Alternative Name: Myosin Light Chain Kinase (MYLK Products)

Synonyms: AAT7 ELISA Kit, KRP ELISA Kit, MLCK ELISA Kit, MLCK1 ELISA Kit, MLCK108 ELISA Kit, MLCK210 ELISA Kit, MSTP083 ELISA Kit, MYLK1 ELISA Kit, smMLCK ELISA Kit, 9530072E15Rik ELISA Kit, A930019C19Rik ELISA Kit, AW489456 ELISA Kit, Mlck ELISA Kit, SMMLCK ELISA Kit, mlck ELISA Kit, fj60a06 ELISA Kit, mlck1a ELISA Kit, mylk ELISA Kit, wu:fj60a06 ELISA Kit, zmlck1 ELISA Kit, myosin light chain kinase ELISA Kit, myosin, light polypeptide kinase ELISA Kit, myosin, light chain kinase a ELISA Kit, MYLK ELISA Kit, Mylk ELISA Kit, mylka ELISA Kit

Background:

Gene Name: Myosin Light Chain Kinase

Gene Aliases: KRP, MLCK108, MLCK210, MSTP083, MLCK, s mMLCK, MYLK1, MLCK1, Telokin, Myosin Light Chain Kinase, Smooth Muscle, deglutamylated form, Kinase-related protein