MYL1 ELISA Kit

Catalog No. ABIN5657103

Product Details MYL1 ELISA Kit

Target: MYL1 (Myosin, Light Chain 1, Alkali, Skeletal, Fast (MYL1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Myosin Light Chain 1, Alkali, Fast Skeletal (MYL1). No significant cross-reactivity or interference between Myosin Light Chain 1, Alkali, Fast Skeletal (MYL1) and analogues was observed.

Sensitivity: 0.056 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Light Chain 1, Alkali, Fast Skeletal (MYL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Light Chain 1, Alkali, Fast Skeletal (MYL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Light Chain 1, Alkali, Fast Skeletal (MYL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myosin Light Chain 1, Alkali, Fast Skeletal (MYL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myosin Light Chain 1, Alkali, Fast Skeletal (MYL1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myosin Light Chain 1, Alkali, Fast Skeletal (MYL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for MYL1

Target: MYL1 (Myosin, Light Chain 1, Alkali, Skeletal, Fast (MYL1))

Alternative Name: Myosin Light Chain 1, Alkali, Fast Skeletal (MYL1 Products)

Synonyms: MLC1F ELISA Kit, MLC3F ELISA Kit, alkali ELISA Kit, fast ELISA Kit, myosin ELISA Kit, skeletal ELISA Kit, AI325107 ELISA Kit, MLC1f ELISA Kit, MLC3f ELISA Kit, Mylf ELISA Kit, MLC3-f ELISA Kit, MLCf ELISA Kit, Mcl3 ELISA Kit, Mlc1 ELISA Kit, Mlc3 ELISA Kit, fj53a11 ELISA Kit, wu:fj53a11 ELISA Kit, zgc:65785 ELISA Kit, zgc:77231 ELISA Kit, MLC1f/3f ELISA Kit, XMLC1f ELISA Kit, XMLC3f ELISA Kit, myl1 ELISA Kit, myosin light chain 1 ELISA Kit, myosin, light chain 1, alkali; skeletal, fast ELISA Kit, myosin, light polypeptide 1 ELISA Kit, myosin, light chain 1 ELISA Kit, Myosin regulatory light chain 1 ELISA Kit, myosin light chain 1 L homeolog ELISA Kit, MYL1 ELISA Kit, Myl1 ELISA Kit, mlc-1 ELISA Kit, myl1 ELISA Kit, myl1.L ELISA Kit

Background:

Gene Name: Myosin Light Chain 1, Alkali, Fast Skeletal

Gene Aliases: MLC1, MLC1F, MLC3F, Myosin light chain 1/3, skeletal muscle isoform, Myosin light chain A1/A2

Gene ID: 4632

UniProt: P05976