Myosin 9 ELISA Kit

Catalog No. ABIN5657102

Product Details Myosin 9 ELISA Kit

Target: Myosin 9 (MYH9)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Myosin Heavy Chain 9, Non Muscle (MYH9). No significant cross-reactivity or interference between Myosin Heavy Chain 9, Non Muscle (MYH9) and analogues was observed.

Sensitivity: 0.057 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Heavy Chain 9, Non Muscle (MYH9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Heavy Chain 9, Non Muscle (MYH9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Heavy Chain 9, Non Muscle (MYH9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myosin Heavy Chain 9, Non Muscle (MYH9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myosin Heavy Chain 9, Non Muscle (MYH9) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myosin Heavy Chain 9, Non Muscle (MYH9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Myosin 9

Target: Myosin 9 (MYH9)

Alternative Name: Myosin Heavy Chain 9, Non Muscle (MYH9 Products)

Synonyms: BDPLT6 ELISA Kit, DFNA17 ELISA Kit, EPSTS ELISA Kit, FTNS ELISA Kit, MHA ELISA Kit, NMHC-II-A ELISA Kit, NMMHC-IIA ELISA Kit, NMMHCA ELISA Kit, C80049 ELISA Kit, D0Jmb2 ELISA Kit, E030044M24Rik ELISA Kit, Fltn ELISA Kit, Myhn-1 ELISA Kit, Myhn1 ELISA Kit, NMHC II-A ELISA Kit, NMHCIIA ELISA Kit, NMMHC II-a ELISA Kit, NMMHC-A ELISA Kit, TU72.6 ELISA Kit, fi22c04 ELISA Kit, myh9 ELISA Kit, myh9l2 ELISA Kit, wu:fi22c04 ELISA Kit, wu:fj85e11 ELISA Kit, zgc:162029 ELISA Kit, zgc:66164 ELISA Kit, NMMHC ELISA Kit, myosin ELISA Kit, non-muscle ELISA Kit, nonmuscle ELISA Kit, KLG/PTK7 ELISA Kit, dfna17 ELISA Kit, epsts ELISA Kit, ftns ELISA Kit, nmhc-ii-a ELISA Kit, nmmhca ELISA Kit, myosin heavy chain 9 ELISA Kit, myosin, heavy polypeptide 9, non-muscle ELISA Kit, myosin, heavy chain 9a, non-muscle ELISA Kit, myosin, heavy chain 9, non-muscle ELISA Kit, myosin, heavy chain 9, non-muscle L homeolog ELISA Kit, MYH9 ELISA Kit, Myh9 ELISA Kit, myh9a ELISA Kit, myh9.L ELISA Kit

Background:

Gene Name: Myosin Heavy Chain 9, Non Muscle

Gene Aliases: DFNA17, EPSTS, FTNS, MHA, NMHC-II-A, NMMHCA, Nonmuscle Myosin Heavy Chain II-A, Cellular myosin heavy chain, type A, Myosin heavy chain, non-muscle IIa

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Integrin Complex