MYH3 ELISA Kit

Catalog No. ABIN5657091

Product Details MYH3 ELISA Kit

Target: MYH3 (Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3). No significant cross-reactivity or interference between Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3) and analogues was observed.

Sensitivity: 0.126 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for MYH3

Target: MYH3 (Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3))

Alternative Name: Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3 Products)

Synonyms: HEMHC ELISA Kit, MYHC-EMB ELISA Kit, MYHSE1 ELISA Kit, SMHCE ELISA Kit, Myhse ELISA Kit, RNMHCG ELISA Kit, MyHC-emb ELISA Kit, Myhs-e ELISA Kit, MHC A ELISA Kit, MYH1B ELISA Kit, MYH3 ELISA Kit, Myosin-1B ELISA Kit, Myosin-3 ELISA Kit, myosin heavy chain 3 ELISA Kit, myosin, heavy polypeptide 3, skeletal muscle, embryonic ELISA Kit, Myosin-3 ELISA Kit, myosin, heavy chain 1D, skeletal muscle ELISA Kit, myosin, heavy chain 3, skeletal muscle, embryonic ELISA Kit, MYH3 ELISA Kit, Myh3 ELISA Kit, myo-3 ELISA Kit, MYH1D ELISA Kit

Background:

Gene Name: Myosin Heavy Chain 3, Skeletal Muscle, Embryonic

Gene Aliases: HEMHC, MYHC-EMB, MYHSE1, SMHCE, Myosin,Skeletal,Heavy chain,Embryonic 1, Myosin heavy chain, fast skeletal muscle, embryonic

Gene ID: 4621

UniProt: P11055