MLH1 ELISA Kit

Catalog No. ABIN5657021

Product Details MLH1 ELISA Kit

Target: MLH1 (MutL Homolog 1 (MLH1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of MutL Homolog 1 (MLH1). No significant cross-reactivity or interference between MutL Homolog 1 (MLH1) and analogues was observed.

Sensitivity: 0.057 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MutL Homolog 1 (MLH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MutL Homolog 1 (MLH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MutL Homolog 1 (MLH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MutL Homolog 1 (MLH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MutL Homolog 1 (MLH1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MutL Homolog 1 (MLH1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for MLH1

Target: MLH1 (MutL Homolog 1 (MLH1))

Alternative Name: MutL Homolog 1 (MLH1 Products)

Synonyms: CG11482 ELISA Kit, Dmel\\CG11482 ELISA Kit, dmlh-1 ELISA Kit, dmlh1 ELISA Kit, zgc:66301 ELISA Kit, MLH1 ELISA Kit, LOC100232198 ELISA Kit, 1110035C23Rik ELISA Kit, AI317206 ELISA Kit, AI325952 ELISA Kit, AI561766 ELISA Kit, COCA2 ELISA Kit, FCC2 ELISA Kit, HNPCC ELISA Kit, HNPCC2 ELISA Kit, hMLH1 ELISA Kit, mutL homolog 1 ELISA Kit, CG11482 gene product from transcript CG11482-RB ELISA Kit, mutL homolog 1 S homeolog ELISA Kit, mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) ELISA Kit, DNA mismatch repair protein Mlh1 ELISA Kit, MLH1 ELISA Kit, Mlh1 ELISA Kit, mlh1.S ELISA Kit, mlh1 ELISA Kit, LOC588545 ELISA Kit

Background:

Gene Name: MutL Homolog 1

Gene Aliases: COCA2, FCC2, HNPCC, HNPCC2, hMLH1, DNA mismatch repair protein Mlh1

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response