SLC25A37 ELISA Kit

Catalog No. ABIN5656919

Product Details SLC25A37 ELISA Kit

Target: SLC25A37 (Solute Carrier Family 25, Member 37 (SLC25A37))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Mitoferrin (MFRN). No significant cross-reactivity or interference between Mitoferrin (MFRN) and analogues was observed.

Sensitivity: 0.053 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mitoferrin (MFRN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mitoferrin (MFRN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mitoferrin (MFRN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mitoferrin (MFRN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mitoferrin (MFRN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mitoferrin (MFRN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for SLC25A37

Target: SLC25A37 (Solute Carrier Family 25, Member 37 (SLC25A37))

Alternative Name: Mitoferrin (SLC25A37 Products)

Synonyms: MFRN ELISA Kit, MFRN1 ELISA Kit, MSC ELISA Kit, MSCP ELISA Kit, PRO1278 ELISA Kit, PRO1584 ELISA Kit, PRO2217 ELISA Kit, 1700020E22Rik ELISA Kit, 4930513O14Rik ELISA Kit, 4930526G11Rik ELISA Kit, AI848481 ELISA Kit, C330015G08Rik ELISA Kit, Mfrn ELISA Kit, Mfrn1 ELISA Kit, Mscp ELISA Kit, frascati ELISA Kit, mitoferrin ELISA Kit, RGD1359361 ELISA Kit, fc73c03 ELISA Kit, wu:fc73c03 ELISA Kit, SLC25A37 ELISA Kit, solute carrier family 25 member 37 ELISA Kit, solute carrier family 25, member 37 ELISA Kit, solute carrier family 25 (mitochondrial iron transporter), member 37 ELISA Kit, solute carrier family 25 (mitochondrial iron transporter), member 37 S homeolog ELISA Kit, SLC25A37 ELISA Kit, Slc25a37 ELISA Kit, slc25a37 ELISA Kit, slc25a37.S ELISA Kit

Background:

Gene Name: Mitoferrin

Gene Aliases: SLC25A37, MSC, HT015, MSCP, Solute Carrier Family 25 Member 37, Mitochondrial iron transporter 1, Mitochondrial solute carrier protein

Gene ID: 51312

UniProt: Q9NYZ2

Pathways: Transition Metal Ion Homeostasis