MAP1A ELISA Kit

Catalog No. ABIN5656889

Product Details MAP1A ELISA Kit

Target: MAP1A (Microtubule-Associated Protein 1A (MAP1A))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Microtubule Associated Protein 1A (MAP1A). No significant cross-reactivity or interference between Microtubule Associated Protein 1A (MAP1A) and analogues was observed.

Sensitivity: 0.128 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Microtubule Associated Protein 1A (MAP1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Microtubule Associated Protein 1A (MAP1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Microtubule Associated Protein 1A (MAP1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Microtubule Associated Protein 1A (MAP1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Microtubule Associated Protein 1A (MAP1A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Microtubule Associated Protein 1A (MAP1A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for MAP1A

Target: MAP1A (Microtubule-Associated Protein 1A (MAP1A))

Alternative Name: Microtubule Associated Protein 1A (MAP1A Products)

Synonyms: Dmel\\CG13630 ELISA Kit, MAP1A ELISA Kit, F4L23.25 ELISA Kit, methionine aminopeptidase 1A ELISA Kit, DKFZp468P0224 ELISA Kit, 6330416M19Rik ELISA Kit, AI853608 ELISA Kit, Mtap-1 ELISA Kit, Mtap1 ELISA Kit, Mtap1a ELISA Kit, moth1 ELISA Kit, MAP1L ELISA Kit, MTAP1A ELISA Kit, CG13630 gene product from transcript CG13630-RA ELISA Kit, microtubule associated protein 1A ELISA Kit, methionine aminopeptidase 1A ELISA Kit, methionine aminopeptidase ELISA Kit, microtubule-associated protein 1 A ELISA Kit, microtubule-associated protein 1A ELISA Kit, CG13630 ELISA Kit, MAP1A ELISA Kit, Map1a ELISA Kit

Gene ID: 25152

UniProt: P34926

Pathways: Microtubule Dynamics, Sensory Perception of Sound