MTAP ELISA Kit

Catalog No. ABIN5656872

Product Details MTAP ELISA Kit

Target: MTAP (Methylthioadenosine phosphorylase (MTAP))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Methylthioadenosine Phosphorylase (MTAP). No significant cross-reactivity or interference between Methylthioadenosine Phosphorylase (MTAP) and analogues was observed.

Sensitivity: 0.063 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Methylthioadenosine Phosphorylase (MTAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Methylthioadenosine Phosphorylase (MTAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Methylthioadenosine Phosphorylase (MTAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Methylthioadenosine Phosphorylase (MTAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Methylthioadenosine Phosphorylase (MTAP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Methylthioadenosine Phosphorylase (MTAP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for MTAP

Target: MTAP (Methylthioadenosine phosphorylase (MTAP))

Alternative Name: Methylthioadenosine Phosphorylase (MTAP Products)

Synonyms: BDMF ELISA Kit, DMSFH ELISA Kit, DMSMFH ELISA Kit, LGMBF ELISA Kit, MSAP ELISA Kit, c86fus ELISA Kit, Cc1-6 ELISA Kit, 1300019I21Rik ELISA Kit, MTAPase ELISA Kit, zgc:66012 ELISA Kit, mtap ELISA Kit, methylthioadenosine phosphorylase ELISA Kit, 5'-methylthioadenosine phosphorylase ELISA Kit, 6-oxopurine nucleoside phosphorylase ELISA Kit, methylthioadenosine phosphorylase L homeolog ELISA Kit, MTAP ELISA Kit, Mtap ELISA Kit, mtaP ELISA Kit, MMAH_RS04260 ELISA Kit, TAGG_RS05050 ELISA Kit, Toce_0364 ELISA Kit, VDIS_RS03165 ELISA Kit, MFER_RS02485 ELISA Kit, Intca_0886 ELISA Kit, DESMU_RS05370 ELISA Kit, Dester_1490 ELISA Kit, ARCVE_RS05445 ELISA Kit, Mahau_1873 ELISA Kit, MZHIL_RS01440 ELISA Kit, mtap ELISA Kit, mtap.L ELISA Kit

Background:

Gene Name: Methylthioadenosine Phosphorylase

Gene Aliases: MSAP, S-methyl-5'-thioadenosine phosphorylase

Gene ID: 4507

UniProt: Q13126

Pathways: Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process